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Evaluation of temporary immersion bioreactors for in vitro micropropagation of banana (Musa spp.) and genetic fidelity assessment using flow cytometry and simple-sequence repeat markers

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Title Evaluation of temporary immersion bioreactors for in vitro micropropagation of banana (Musa spp.) and genetic fidelity assessment using flow cytometry and simple-sequence repeat markers
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Creator S. Uma, R. Karthic, S.Kalpana, S. Backiyarani
 
Subject (TIB) technology, benzylaminopurine, naphthaleneacetic acid, chlorophyll pigment, flow cytometry
 
Description Banana is a pioneer crop in commercial application of in vitro shoot tip culture techniques for large-scale propagation of its planting material (Arun Waman and Bohra, 2018). The increasing demand for planting material and the need for higher productivity have led to the development of advanced technologies. Commercial laboratories are seeking cost-efficient technologies to reduce overhead and production costs (Mazri et al., 2021; Cardoso et al., 2018; Kodym and Zapata-Arias, 2001). The current shoot tip culture technique is often criticized due to low multiplication potential, long multiplication process coupled with intensive labor requirements (Chen, 2016; Aitken-Christie et al., 1995; Chu, 1995; Pachauri and Dhawan, 1989). The efficiency of in vitro shoot tip culture is determined as multiplication rate based on induction of maximum number of shoots per explant during periodical subcultures, which depends mainly on nutrients supplemented in the growing media and the form of supplementation.
Temporary immersion bioreactor (TIB) technology offers a cost-effective means of in vitro micropropagation for large-scale plantlet production, and is increasingly being recognized as a valuable tool in the plant tissue culture industry. However, the limited accessibility of the bioreactor in certain markets is hindering its potential benefits. Here, we report three different TIB systems for large-scale plantlet production in banana. Shoot tip explants of commercially important banana cultivars such as Grand Naine (AAA), Red Banana (AAA), Nendran (AAB), and Ney Poovan (AB) were initiated aseptically in semisolid medium and cultured in the TIB systems using liquid medium supplemented with 4.0 mg/l 6- benzylaminopurine (BAP), 0.2 mg/l indole-3-acetic acid (IAA), and 30 g/l sucrose for shoot multiplication and liquid medium supplemented with 1.0 mg/l indole-3-butyric acid (IBA) and 2.0 mg/l 1-naphthaleneacetic acid (NAA) for rooting of shoots. The liquid media were supplied periodically (2 min for every 6-hr frequency) to the growth vessels and recorded the data on number of shoots per explant, shoot length and number of roots per shoot. The data were compared with explants cultured in semisolid medium. The three TIBs showed superior response over semisolid culture system in shoots per explant, shoot length and number of roots per shoot. There was no significant difference within TIBs in photosynthetic efficiency as measured by chlorophyll pigment analysis. Plantlets cultured in TIBs showed better survival under ex vitro conditions. The genetic integrity of the plantlets grown in TIBs were confirmed by flow cytometry and simple-sequence repeat (SSR) markers analysis. The developed TIBs and protocol can be utilized for commercial purpose.
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Date 2024-04-08T09:58:05Z
2024-04-08T09:58:05Z
2023-04-22
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/81894
 
Language English
 
Relation Not Available;
 
Publisher South African Journal of Botany