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<em>In vitro</em> validation of anti-diabetic and free radical scavenging property of <em>Naaval Kottai Mathirai</em>, a promising Siddha drug for the management of Diabetes mellitus

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Title Statement <em>In vitro</em> validation of anti-diabetic and free radical scavenging property of <em>Naaval Kottai Mathirai</em>, a promising Siddha drug for the management of Diabetes mellitus
 
Added Entry - Uncontrolled Name S, Sivakkumar ; National Institute of Siddha, Ministry of Ayush, Chennai 600 047, India
 
Uncontrolled Index Term Anti-diabetic assay, Anti-oxidant assay, Naval Kottai Mathirai, Siddha medicine
 
Summary, etc. The World Health Organization (WHO) reported 422 million total Diabetic population worldwide and about 1.5 million death rates caused due to it. Diabetes Mellitus (<em>Madhumegam</em>) is a disease classified under <em>Neerinai Perkukkal Noi</em> (diseases causing excessive urination) caused due to increased <em>kapam humour</em>. <em>Naaval Kottai Mathirai </em>(NKM) is a unique herbal Siddha drug prepared from <em>Aristolochia bracteolata</em> which has bitter taste and <em>Syzygium cumini </em>which has astringent taste. The herbal formulation NKM neutralizes <em>kapam</em> and hence effective in <em>Madhumegam</em>. <em>In vitro</em> studies like alpha-amylase, alpha-glucosidase inhibitory assays were performed to prove the anti diabetic effect of the drug NKM. DPPH (2,2- Diphenyl 1-2 picrylhydrazyl) assay, hydrogen peroxide scavenging assay (H₂O₂) and nitric oxide radical scavenging assay were done to evaluate its antioxidant potency. The results showed that minimum alpha amylase inhibitory activity ranged from 19.42±5.92 to 60.92±3.98%, with an IC<sub>50</sub> value 70.9±12.37 μg/mL, when it is compared with standard drug acarbose, which showed inhibition ranged from 39.83±2.18 to 91.08±5.43 with an IC<sub>50</sub> value 13.29±1.95 μg/mL in various concentration levels of NKM. The IC<sub>50</sub> value of Butylated Hydroxyanisole (BHA) was noted as 48.61±1.69 μg/mL and NKM as 67.19±7.81 μg/mL for the H<sub>2</sub>O<sub>2</sub> scavenging antioxidant activity. In nitrogen scavenging antioxidant activity, it was observed that IC<sub>50</sub> value of gallic acid was 23.02 μg/mL and NKM was 79.13 μg/mL. In DPPH scavenging activity, for ascorbic acid, the standard showed its IC<sub>50</sub> value as 13.36 μg/mL and NKM as 53.39 μg/mL. The phytoconstituents present in NKM may enrich the antidiabetic and antioxidant properties of the drug.
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2024-04-18 12:47:04
 
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http://op.niscair.res.in/index.php/IJTK/article/view/72534
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 23, ##issue.no## 3 (2024): Indian Journal of Tradeitional Knowledge
 
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