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Vitex negundo L. leaf extract suppresses the cell cycle and promotes apoptosis in colon cancer cells

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Title Vitex negundo L. leaf extract suppresses the cell cycle and promotes apoptosis in colon cancer cells
 
Creator Gouthami, Kuruvalli
L, Lavanya
Pulakuntla, Swetha
CN, Prashantha
Shaik, Althaf Hussain
Reddyvari, Hymavathi
Vadamalai, Veeraraghavan
Reddy, Vaddi Damodara
 
Subject Antioxidant activity
Colorectal cancer
HCT116-colon cancer cells
Vitex negundo L.
 
Description 269-278
Colon cancer has become a frequent malignancy worldwide. Vitex negundo (V. negundo L.), is an herb used in traditional
medicine to treat many kinds of diseases. This study investigated the molecular mechanism involved in colon cancer
prevention using human colon cancer (HCT-116) cell lines with methanolic leaf extract of V. negundo L. DPPH and NOX
radical scavenging properties, cell cycle analyses, Annexin V-FITC/PI, MTT assay, and qRT-PCR were utilized to
determine Vitex negundo's impact on antioxidant properties, cytotoxicity, cell cycle and apoptosis in HCT-116 cells. Our
results revealed that V. negundo L. exhibited strong DPPH and nitric oxide radical scavenging properties in a dose-related
manner. Additionally, the extract inhibits cell growth in a way that is dependent on both time and dose. The IC50 was found
to be 54 μg/mL. Annexin V-FITC/PI confirmed apoptosis induction. Furthermore, the cell cycle studies demonstrated that
the arrest occurred during the G2/M phase. The essential appliance of G2/M arrest is believed to be related to CDK1,
survivin and cyclin B1 gene downregulation, as confirmed by qRT-PCR. In conclusion, our findings show that V. negundo
L. extract has excellent antioxidant properties, suppresses the cell cycle, and promotes apoptosis.
 
Date 2024-04-18T05:34:26Z
2024-04-18T05:34:26Z
2024-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/63785
https://doi.org/10.56042/ijbb.v61i5.2015
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.61(05) [May 2024]