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Caesalpinia bonducella seeds restore the ovarian functions in mifepristoneinduced PCOS rats acting through insulin –insulin-like growth factors

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Title Caesalpinia bonducella seeds restore the ovarian functions in mifepristoneinduced PCOS rats acting through insulin –insulin-like growth factors
 
Creator Murugesan, Meera
Hari, Rajeswary
Muralidharan, P
Durairaj, Priya
Sekar, Karthikeyan
 
Subject IGF-1
IGF-2
IRS1
IRS2
PCOS
PTEN
 
Description 381-390
The prime complications namely hyperandrogenism and hyperinsulinemia, observed in PCOS condition is due to the
abnormal signalling pathways triggered by insulin as well as insulin-like growth factor family of genes and proteins. The
aim of the present study is to evaluate the down regulating effect of ethanolic seed extract of Caesalpinia bonducella
(ESECB) on the genes like IGF-1, IGF-2 and PTEN and proteins IRS1 and IRS2 in mifepristone induced PCOS rats. The
level expression pattern of genes were studied using in vivo methods and the in silico method is used to study the proteins.
Mifepristone induced PCOS rats were treated with 200 mg and 400 mg/kg b.w. p.o of ESECB extract for 28 days. The
ovaries were collected for gene expression study using semi quantitative real time PCR analysis. Molecular docking analysis
was performed from the GC-MS phyto-constituents for a antagonistic ligand which can halt the reactions of IRS1 and IRS2
proteins. The fold change of the mRNA expression of IGF-1, IGF-2 and PTEN genes were found to be 1.4, 0.34 and 0.36
respectively in the mifepristone induced PCOS rat ovaries. ESECB treatment decreased the fold change to 0.44, 0.13 and 0,
20 respectively. In docking studies, eight ligands namely, dioxan, propyl acetate, acetic anhydride, methyl butenoic acid,
methyl isopropyl carbonate, glycerine, diethanolamine and 2,2, dimethyl propanoic anhydride inhibted the IRS1 and IRS2
proteins by interacting with their amino acid residues. It can be concluded that ESECB extract can be used as a potential
drug for the treatment of PCOS as it acts at the molecular level to correct the complications of this disease.
 
Date 2024-04-18T06:18:18Z
2024-04-18T06:18:18Z
2024-04
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/63790
https://doi.org/10.56042/ijtk.v23i4.10331
 
Language en
 
Relation Int Cl.24: A61K 36/00
 
Publisher NIScPR-CSIR,India
 
Source IJTK Vol.23(4) [April 2024]