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Expression Dynamics of lpa1 Gene and Accumulation Pattern of Phytate in Maize Genotypes Possessing opaque2 and crtRB1 Genes at Different Stages of Kernel Development

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Title Expression Dynamics of lpa1 Gene and Accumulation Pattern of Phytate in Maize Genotypes Possessing opaque2 and crtRB1 Genes at Different Stages of Kernel Development
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Creator Vinay Bhatt 1,2 , Vignesh Muthusamy 1,* , Kusuma Kumari Panda 2 , Ashvinkumar Katral 1 , Rashmi Chhabra 1, Subhra J. Mishra 1 , Ikkurti Gopinath 1, Rajkumar U. Zunjare 1, Chirravuri Naga Neeraja 3 , Sujay Rakshit 4,5, Devendra K. Yadava 1 and Firoz Hossain 1
 
Subject low phytic acid; expression; gene transcript; minerals; Zea mays
 
Description Not Available
Phytic acid (PA) acts as a storehouse for the majority of the mineral phosphorous (P) in
maize; ~80% of the total P stored as phytate P is not available to monogastric animals and thereby
causes eutrophication. In addition, phytic acid chelates positively charged minerals making them
unavailable in the diet. The mutant lpa1-1 allele reduces PA more than the wild-type LPA1 allele.
Further, mutant gene opaque2 (o2) enhances lysine and tryptophan and crtRB1 enhances provitamin-A
(proA) more than wild-type O2 and CRTRB1 alleles, respectively. So far, the expression pattern of
the mutant lpa1-1 allele has not been analysed in maize genotypes rich in lysine, tryptophan and
proA. Here, we analysed the expression pattern of wild and mutant alleles of LPA1, O2 and CRTRB1
genes in inbreds with (i) mutant lpa1-1, o2 and crtRB1 alleles, (ii) wild-type LPA1 allele and mutant
o2 and crtRB1 alleles and (iii) wild-type LPA1, O2 and CRTRB1 alleles at 15, 30 and 45 days after
pollination (DAP). The average reduction of PA/total phosphorous (TP) in lpa1-1 mutant inbreds
was 29.30% over wild-type LPA1 allele. The o2 and crtRB1-based inbreds possessed ~two-fold higher
amounts of lysine and tryptophan, and four-fold higher amounts of proA compared to wild-type
alleles. The transcript levels of lpa1-1, o2 and crtRB1 genes in lpa1-1-based inbreds were significantly
lower than their wild-type versions across kernel development. The lpa1-1, o2 and crtRB1 genes
reached their highest peak at 15 DAP. The correlation of transcript levels of lpa1-1 was positive for
PA/TP (r = 0.980), whereas it was negative with inorganic phosphorous (iP) (r = 􀀀0.950). The o2 and
crtRB1 transcripts showed negative correlations with lysine (r = 􀀀0.887) and tryptophan (r = 􀀀0.893),
and proA (r = 􀀀0.940), respectively. This is the first comprehensive study on lpa1-1 expression in the
maize inbreds during different kernel development stages. The information generated here offers
great potential for comprehending the dynamics of phytic acid regulation in maize.
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Date 2024-04-20T12:45:16Z
2024-04-20T12:45:16Z
2023-04-24
 
Type Article
 
Identifier Bhatt, V.; Muthusamy, V.; Panda, K.K.; Katral, A.; Chhabra, R.; Mishra, S.J.; Gopinath, I.; Zunjare, R.U.; Neeraja, C.N.; Rakshit, S.; et al. Expression Dynamics of lpa1 Gene and Accumulation Pattern of Phytate in Maize Genotypes Possessing opaque2 and crtRB1 Genes at Different Stages of Kernel Development. Plants 2023, 12, 1745. https://doi.org/ 10.3390/plants12091745
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http://krishi.icar.gov.in/jspui/handle/123456789/82253
 
Language English
 
Relation Not Available;
 
Publisher MDPI