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Determination of lethal dose (LD50) and sensitivity of fenugreek (Trigonella foenum-graecum) to sodium azide for induction of mutation

Indian Agricultural Research Journals

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Title Determination of lethal dose (LD50) and sensitivity of fenugreek (Trigonella foenum-graecum) to sodium azide for induction of mutation
 
Creator KHAN, RUBINA
PUNIA, S S
DALIP
RAM, MANOHAR
GUPTA, DEEPAK
BHATT, BABITA
AHMAD, SARFRAZ
DHEER, MEENAKSHI
RAJPUT, S S
KUMAWAT, G L
 
Subject Fenugreek, Induced mutation, Lethal dose, Sodium azide
 
Description The present experiment was conducted in the year 2021 at Sri Karan Narendra Agriculture University, Jobner, Rajasthan, to determine the optimum dose (LD50) and duration of the chemical mutagen treatment for the induction of desirable mutation. A set of 100 pre-soaked fenugreek seeds were treated with 12 different concentrations of sodium azide, viz. 0.3 mM, 0.6 mM, 1 mM, 2 mM, 3 mM,
4 mM, 5 mM, 6 mM, 7 mM, 8 mM, 9 mM and 10 mM for three different durations, viz. 3 h, 6 h and 9 h. Results showed that a dose-dependent decreasing tendency was observed in germination percentage, survival percentage, root length and shoot length with increasing concentration and duration of sodium azide treatment. Almost all the mutagenic treatments resulted in decrease in germination percentage, survival percentage and seedling height (root and shoot length) with increasing concentrations and duration of mutagen in laboratory conditions. Also, the LD50 value was observed as an 8 mM sodium azide concentration for 6 h in fenugreek. The mutagen treatments given at 9 h duration were detrimental for fenugreek. They cannot be used for mutation induction as they are utterly lethal after a 3 mM sodium azide concentration. So, lower treatments of mutagens have influenced less biological damage and would be suitable for inducing desirable mutations.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-04-24
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAgS/article/view/134877
10.56093/ijas.v94i4.134877
 
Source The Indian Journal of Agricultural Sciences; Vol. 94 No. 4 (2024); 440–443
2394-3319
0019-5022
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAgS/article/view/134877/54380
 
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