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Partial characterization of novel nematicidal toxins from Bacillus cereus Frankland and Frankland 1887 and their effect on root-knot nematode, Meloidogyne incognita(Kofoid& White) Chitwood

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Title Partial characterization of novel nematicidal toxins from Bacillus cereus Frankland and Frankland 1887 and their effect on root-knot nematode, Meloidogyne incognita(Kofoid& White) Chitwood
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Creator M. NAGESH, R. ASOKAN and K. S. MOHAN
 
Subject Bacillus cereus, Meloidogyne incognita, nematicidal toxins, partial characterization
 
Description Not Available
Bacillus cereus isolated from the egg masses of root-knot nematode,
MeIoidogyne incognita from tomato rhizosphere was grown in casein-peptone soy meal peptone
(CASO) broth for partial characterization of toxins from cell-free filtrates. Cell-free culture
filtrates of B. cereus at 72 hours of growth and beyond, reduced egg hatching (90'l"o) and caused
100 per cent mdrtality of juveniles in 4 hours of exposure, which coincided with the postsporulation phase (72h) of the bacterial growth indicating profound toxicity during and after
the sporulation phase. The native PAGE electrophoresis of the partially purified cell-free
culture fiitrates showed that 3 bands of::::: 15, 40 and 60 kDa appeared in 72, 84 and 96 hours of
growth, respectively, coinciding with post-sporulation phase of the bacterium.
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Date 2024-04-24T10:07:04Z
2024-04-24T10:07:04Z
2005-05-15
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/82307
 
Language English
 
Relation Not Available;
 
Publisher Not Available