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PCR amplification, sequencing, and in silico analysis of holandric genes TSPYand SRY in Nili Ravi buffalo

Indian Agricultural Research Journals

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Title PCR amplification, sequencing, and in silico analysis of holandric genes TSPYand SRY in Nili Ravi buffalo
 
Creator SINGH, SANDEEP
SINGH, RANBIR
MUKHOPADHYAY, C S
 
Subject Evolution, Nili Ravi, SRY, Structure prediction, TSPY, Y-Chromosome
 
Description The present work has been designed to study the evolutionary perspectives of two important holandric genes, namely, testis-specific protein, Y-encoded (TSPY), and sex determining region, Y-encoded (SRY) in Nili Ravi buffalo. These Y-chromosomal genes are responsible for male sex development. The evolutionary aspects of these genes have not been studied in the Nili Ravi buffaloes from India. A study was carried out on the peripheral blood samples of three adult Nili Ravi bulls belonging to university farm. DNA was extracted using the phenol-chloroform isoamyl alcohol (PCI) technique and reported primer sequences were used to amplify the genes (partially) using a thermocycler. Three biological samples were analyzed using Sanger sequencing and were aligned using Clustal Omega. Separate phylogenetic trees for the genes were constructed using MEGA7 software. The selection pressure analysis and evolutionary divergence were individually studied for each of the genes. The current study reports the partial TSPY and SRY gene sequences of Indian Nili Ravi buffalo. Besides, the molecular evolution analysis indicates that the genes have experienced some purifying selection during the evolutionary process. Molecular characterization of these holandric genes (TSPY and SRY) in Nili Ravi will find applications in sex determination in Nili Ravi and other buffaloes.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-05-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/123881
10.56093/ijans.v94i5.123881
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 5 (2024); 426–430
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/123881/54457
https://epubs.icar.org.in/index.php/IJAnS/article/view/123881/54458
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0