Comparative sensitivity of three new cell lines developed from gill, liver and brain tissues of goldfish, Carassius auratus (L.) to cyprinid herpesvirus-2 (CyHV-2).
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Title |
Comparative sensitivity of three new cell lines developed from gill, liver and brain tissues of goldfish, Carassius auratus (L.) to cyprinid herpesvirus-2 (CyHV-2).
Not Available |
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Creator |
Swaminathan T.R.
Arun Raja Arathi Dharmaratnam Sundar R.N. |
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Subject |
Carassius auratus
Cyprinid herpesvirus-2 (CyHV-2) Gill Liver Brain Cell line |
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Description |
New cell lines were established from the gill, liver and brain of goldfish and they were denoted as Fantail goldfish gill (FtGG), Fantail goldfish liver (FtGL) and Fantail goldfish brain (FtGB) and their capability to replicate CyHV-2 was evaluated by virus infection and titration.
Cyprinid herpesvirus 2 (CyHV-2) is the etiological agent of Goldfish herpesviral haematopoietic necrosis (GHVHN) in goldfish. In this study, three new cell lines including Fantail goldfish gill (FtGG), Fantail goldfish liver (FtGL) and Fantail goldfish brain (FtGB) had been established and characterized from the gill, liver and brain tissue of C. auratus respectively. Cell lines were optimally grown at 28 ◦C in Leibovitz-15 (L-15) medium supplemented with 10 % fetal bovine serum (FBS). The PDT during exponential growth of FtGG, FtGL and FtGB cells were determined to be 41.47 h, 63.43 h and 79.79 h respectively. Karyotyping analysis of cell lines remained diploid (2n = 100). The revival rate was 82 %, 72 % and 70 % in FtGG, FtGL and FtGB cells respectively after 6 months of cryopreservation. All the three cells showed similar cytopathic effect (CPE) between 3–5 days post infection (dpi) with CyHV-2 and complete destruction of the monolayer was observed at 8–10 dpi. The viral titers of CyHV-2 in FtGG, FtGL and FtGB reached 107.375±0.35 TCID50 ml− 1, 104 55±0.070 TCID50 ml− 1 and 106.45±0.070 TCID50 ml− 1 respectively. These newly established cell lines will be a useful diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in future. ICAR National Fellow Scheme of ICAR (ICAR letter no F.No Agri.edn.27/ 2/2015 HRD dated 13.02.2017), Department of Agricultural Research and Education, Ministry of Agriculture and Farmer’s Welfare, Government of India. |
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Date |
2024-05-02T17:02:31Z
2024-05-02T17:02:31Z 2021-02-05 |
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Type |
Research Paper
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Identifier |
Thangaraj Raja Swaminathan, Arun Raja, Arathi Dharmaratnam, Sundar Raj Nithianantham, Comparative sensitivity of three new cell lines developed from gill, liver and brain tissues of goldfish, Carassius auratus (L.) to cyprinid herpesvirus-2 (CyHV-2), Journal of Virological Methods, Volume 291, 2021, 114069, ISSN 0166-0934, https://doi.org/10.1016/j.jviromet.2021.114069.
0166-0934 http://krishi.icar.gov.in/jspui/handle/123456789/82575 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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