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Immune gene expression in cyprinid herpesvirus-2 (CyHV-2)–sensitized peripheral blood leukocytes (PBLs) co-cultured with CyHV-2-infected goldfish fin cell line

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Title Immune gene expression in cyprinid herpesvirus-2 (CyHV-2)–sensitized peripheral blood leukocytes (PBLs) co-cultured with CyHV-2-infected goldfish fin cell line
Not Available
 
Creator Sweta Das,
Arathi Dharmaratnam
Ravi, C.
Swaminathan T.R.
 
Subject Goldfish
Cyprinid herpesvirus-2
Peripheral blood leukocytes
Immune gene expression
 
Description We checked expression of some of the signature genes (CD8α, IFNγ, b2m, MHC I, LMP 7, IL-10, IL-12, GATA3 and Tapasin) for T-helper cell response to CyHV-2 in sensitized goldfish peripheral blood leukocytes (PBL) against CyHV-2-infected FtGF cells.
Goldfish is one of the preferred ornamental fish which is highly susceptible to cyprinid
herpesvirus-2 (CyHV-2) infection. The present study aimed to analyse immune gene
expression in a co-culture of CyHV-2-sensitized goldfish peripheral blood leukocytes
(PBLs) with CyHV-2-infected fantail goldfish fin cell lines (FtGF). Goldfish were
sensitized with intraperitoneal TCID50 dose (107.8±0.26/mL) of CyHV-2. After 2 weeks,
PBLs were collected and co-cultured with CyHV-2-infected FtGF cells keeping both
uninfected FtGF cells and PBL control groups. After 2 days of co-culture, WST-1 assay
for cell proliferation was performed at 450 nm during the 2nd, 4th and 6th days of co culture. The results showed a significant increase (p < 0.05) in cell density in CyHV-2-
infected PBL and virus-infected FtGF cells during the 4th day post co-culture which
confirmed effector cell generation. Expressions of few immune genes were checked
taking RNA samples of CyHV-2-induced PBLs post co-culture with infected FtGF cells
along with uninfected FtGF cells as control group at different time periods (2nd, 4th and
6th days) in triplicate. The results indicated increased expression of CD8α, IFNγ, b2m,
MHC I, LMP 7, IL-10, IL-12 and GATA3 except Tapasin. From the above study, we
concluded that goldfish showed both Th1- and Th2-mediated immune responses to
CyHV-2. The current findings support the scope for further vaccine development against
CyHV-2 for goldfish.
e Science and Engineering Research Board (SERB), Government of India under the National Postdoctoral Fellowship Scheme (NPDF) (PDF/2016/000066 Dated 07-Dec-2016).
 
Date 2024-05-02T17:09:34Z
2024-05-02T17:09:34Z
2021-07-26
 
Type Research Paper
 
Identifier Das, S., Dharmaratnam, A., Ravi, C. et al. Immune gene expression in cyprinid herpesvirus-2 (CyHV-2)–sensitized peripheral blood leukocytes (PBLs) co-cultured with CyHV-2-infected goldfish fin cell line. Aquacult Int (2021). https://doi.org/10.1007/s10499-021-00721-6;
0967-6120
http://krishi.icar.gov.in/jspui/handle/123456789/82579
 
Language English
 
Relation Not Available;
 
Publisher Springer