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Correlations between beclin-1 and transforming growth factor-β1 in letrozole induced polycystic ovary syndrome

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Title Correlations between beclin-1 and transforming growth factor-β1 in letrozole induced polycystic ovary syndrome
 
Creator Hamdy, Nermin M.
Ghanem, Hala M.
Mohamed, Mervat M.
Abd El-Rahman, Fawzia A.A.
Soliman, Ahmed F.
 
Subject Autophagy
Estradiol
Follicle-stimulating hormone
Luteinizing hormone
Oxidative stress
Progesterone
Protein carbonyl content
Reactive oxygen species (ROS)
Testosterone
Total antioxidant capacity
 
Description 332-340
In the pathogenesis of polycystic ovary syndrome (PCOS), despite the importance of autophagy and transforming growth
factor-β1 (TGF-β1), there is scarce information about their inter-relationship. Therefore, here we assessed the correlations
between beclin-1, a cornerstone in autophagy, and TGF-β1 in a letrozole-induced PCOS rat model. Accordingly, a total of
45 female adult albino Wistar rats were randomly assigned into control, vehicle (carboxymethyl cellulose), and PCO groups.
To establish the PCOS model, letrozole (1.0 mg/kg body wt., p.o.) was given once daily for three successive weeks.
Circulating levels of luteinizing hormone, follicle-stimulating hormone, testosterone, estradiol, and progesterone were
assayed along with ovarian total antioxidant capacity (TAC), protein carbonyl content (PCC), beclin-1 level, and TGF-β1
level. Ovarian morphology and ultrastructure were examined by hematoxylin and eosin staining and electron microscopy,
respectively. Compared to control groups, hormonal levels and ovarian morphology in the letrozole-exposed animals
indicated the successful construction of the PCOS model. Further, the PCO group exhibited an oxidative stress status
reflected by a significant decrease in ovarian TAC and a significant elevation in the PCC. Moreover, ovarian beclin-1 and
TGF-β1 levels were significantly increased with an enhancement of autophagy as revealed by electron microscopy. In
multiple linear regression models, only TGF-β1 was observed in the final model where it explained the 62.3% variability of
Beclin-1. In conclusion, the ovarian level of TGF-β1 might be a determinant factor of beclin-1 level in PCOS which may
provide new insight into the pathophysiology and therapy of the disease.
 
Date 2024-04-29T04:51:26Z
2024-04-29T04:51:26Z
2024-05
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/63826
https://doi.org/10.56042/ijeb.v62i05.1768
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.62(05) [May 2024]