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Genetic polymorphism of follicle stimulating hormone beta (FSHβ) gene and its association with body weight, scrotal measurements and seminal quality traits in indigenous goats

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Title Genetic polymorphism of follicle stimulating hormone beta (FSHβ) gene and its association with body weight, scrotal measurements and seminal quality traits in indigenous goats
 
Creator Khan, Asad
Thakur, Mohan Singh
Joshi, Shrikant
Shukla, Satya Nidhi
Khare, Ankur
Khare, Vaishali
Vandre, Rajesh Kumar
Sharma, Mona
 
Subject Barbari
Black Bengal
Buck fertility
Livestock
Polymorphism
Sirohi
 
Description 309-316
In goat farming, buck fertility is important because most farms typically retain one buck for every few dams. Follicle
stimulating hormone is essential for regulating fertility and varies in its beta subunit (FSHβ), which provides specificity. To
explore the possibilities of using FSHβ gene as candidate marker, this study was aimed to investigate polymorphism at
FSHβ gene by PCR-RFLP assay and association of polymorphic variants of FSHβ with body weights, scrotal and seminal
attributes in indigenous goats. The amplicons of 313 bp was obtained for FSHβ gene from the DNA of 35 Barbari, 26 Black
Bengal, and 31 Sirohi bucks, respectively. FSHβ was found to be monomorphic in all three breeds using PCR-RFLP
analysis. Using PstI and HinfI restriction enzymes, respectively, it revealed a single type of uncut banding pattern of 313 bp
and single fragment of 157 bp. Sequence analysis also confirmed absence of PstI recognition site, revealing an A>G
substitution at nucleotide position 4531. Frequency of AA genotype and A allele was found to be maximum. All traits, with
the exception of mass motility, showed a significant breed effect. Based on the findings, it was concluded that targeted
region of FSHβ gene was fixed goat population under investigation.
 
Date 2024-04-29T05:03:42Z
2024-04-29T05:03:42Z
2024-05
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/63829
https://doi.org/10.56042/ijeb.v62i05.4242
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.62(05) [May 2024]