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Microscope Assisted Uni‑algal isolation through Dilution (MAU‑D): a simple modified technique for tapping diverse cyanobacteria

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Title Microscope Assisted Uni‑algal isolation through Dilution (MAU‑D): a simple modified technique for tapping diverse cyanobacteria
Not Available
 
Creator Verma, S., Bagul, S. Y., Choudhary, P., Chakdar, H., Das, S., Siddiqui, N. and Saxena, A., K
 
Subject Cyanobacteria · Diversity · Isolation · Microscopy · Purif catio
 
Description Not Available
Cyanobacteria are oxygenic photosynthetic microorganisms known for their agricultural and industrial importance. Unavailability
of e cient and fast isolation and purif cation methods of cyanobacteria has impeded our understanding of cyanobacterial
diversity. A number of techniques for isolation and purif cation of cyanobacteria are available, but most of them
are cumbersome as well as time-consuming. In the present study, we modif ed and validated a uni-algal isolation technique
named as Microscope Assisted Uni-algal isolation through Dilution (MAU-D) which used dilution of mixed algal population
on slide and isolation of single type of cyanobacterial cells using light microscope. Using this technique, we obtained
81 cyanobacterial isolates belonging to various species from 19 dif erent genera from soil and water samples collected from
rice f elds of Uttar Pradesh, India. This technique also resulted in isolation of six distinct genera, viz., Cyanobacterium,
Toxopsis, Desertif lum,
Chroococcidiopsis,
Halomicronema, and
Alkalinema,
which were previously not reported from rice
f elds of India. Hence, the MAU-D technique presents a simple, comparatively fast method of isolation and purif cation of
cyanobacteria which can help to isolate those cyanobacteria which are di cult to isolate through routine sub-culturing
Not Available
 
Date 2024-05-10T17:59:44Z
2024-05-10T17:59:44Z
2021-06-17
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/82799
 
Language English
 
Relation Not Available;
 
Publisher Springer