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Antimicrobial activity of the crude peptide extracts from Blackfin sea catfish Arius jella Day, 1877

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Title Antimicrobial activity of the crude peptide extracts from Blackfin sea catfish Arius jella Day, 1877
 
Creator Anju, M V
Reshma, C R
Archana, K
Adarsh, P P
Antony, A
 
Subject Antimicrobial peptides
Arius jella
Crude peptide
Host defence peptides
Innate immunity
 
Description 371-380
Most Antimicrobial Peptides (AMPs) reported from fish possess antimicrobial activity. The study reports the
antibacterial potential of the crude peptide extract from Arius jella, Blackfin sea catfish. The crude peptide extract was
purified by a three-step process, namely acetic acid-acetone precipitation, Sep-pak®C-18 solid-phase purification, and cation
exchange chromatography. The disc diffusion assay revealed that the crude extract obtained through the modified acetic
acid-acetone precipitation method exhibited antimicrobial activity against both Gram-negative and Gram-positive bacteria in
all tested cases. The Sep-Pak purification yielded 5, 40, and 80 % fractions of the peptide, and these fractions underwent
cation exchange chromatography. The peptide fractions (purified) were further tested for antimicrobial activity by broth
microdilution assay. The fractions Aj5-5, Aj40-4, and Aj80-2 showed the highest activity against Bacillus cereus; Aj5-5,
Aj40-5, and Aj80-2 against Vibrio alginolyticus; and Aj5-1, Aj40-1, and Aj80-4 showed highest activity against
Staphylococcus aureus. All the fractions tested were found to be potent against at least one of the pathogens tested. This is
the first report of the isolation of AMPs from Arius jella. Further purification and characterization of the peptides could
potentially unveil novel therapeutic agents to combat pathogens and would be a substantial contribution to the field of
aquaculture and human health management.
 
Date 2024-05-16T06:48:50Z
2024-05-16T06:48:50Z
2024-05
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/63910
https://doi.org/10.56042/ijms.v52i08.11020
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJMS Vol.52(08) [August 2023]