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Detection of Escherichia fergusonii - an emerging pathogen harbouring drug resistant genes from seafood samples of Tamil Nadu, India

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Title Detection of Escherichia fergusonii - an emerging pathogen harbouring drug resistant genes from seafood samples of Tamil Nadu, India
 
Creator Chrisolite, B
Sugumar, G
Sivakumar, S
Mercy, A Angela
 
Subject Antibiotic resistance
Drug resistance
ESBL
Escherichia fergusonii
Public health
Seafood
 
Description 363-370
Escherichia fergusonii harbouring drug resistant genes was isolated from seafood samples of Tamil Nadu, India while
attempting to isolate Escherichia coli. Isolation of STEC was done by enrichment and plating on MacConkey agar MUG
and sorbitol negative colonies were confirmed by IMViC test and by an automated system (Vitek-2 compact, BioMerieux,
France). Antibiotic sensitivity test was performed by disc diffusion method and by PCR for antibiotic resistance genes. A
total of 302 presumptive E. coli was isolated, of which 126 isolates were confirmed as E. coli. Few isolates could not
ferment sorbitol and produce β-glucuronidase, and these were confirmed as presumptive E. coli O157:H7. However, two
isolates were positive for adonitol and therefore confirmed as E. fergusonii (E011 and E060). The two isolates were sorbitol
and lactose negative and adonitol, amygdalin and cellobiose positive. The isolates could not produce the enzyme
β-glucuronidase. The isolates were then confirmed as E. fergusonii by gene specific PCR assay and 16S rDNA sequencing.
ESBL studies showed that both the isolates harbour blaTEM. Presence of drug resistance and ESBL in E. fergusonii isolated
from seafood samples is a matter of public health concern, as the resistance could enter the food chain and hinder the
effectiveness of drugs used for treatment. To the best of our knowledge, this is the first report on the occurrence of
E. fergusonii harbouring drug resistance genes from seafood samples of Tamil Nadu, India.
 
Date 2024-05-16T06:51:38Z
2024-05-16T06:51:38Z
2024-05
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/63911
https://doi.org/10.56042/ijms.v52i08.11019
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJMS Vol.52(08) [August 2023]