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Detection of lytic phage infecting flavour-producing strain of Lacticaseibacillus paracasei in the dairy effluents of Kerala

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Title Detection of lytic phage infecting flavour-producing strain of Lacticaseibacillus paracasei in the dairy effluents of Kerala
Not Available
 
Creator Archana, C.
Beena, A. K.
Murugadas, V.
Lijo John
Rajakumar, S. N.
Sudheer Babu
Ligimol James
Rahila, M. P.
 
Subject Bacteriophage
Dahi
dairy effluent
fermented milk
Lacticaseibacillus paracasei
 
Description Not Available
The performance of the starter culture is a critical factor that decides the quality of fermented milk. Dahi is a fermented milk product popular in India made using a mixed starter culture of lactic acid bacteria comprising acid and flavour producers. The prevalence of bacteriophages in the dairy environment can critically affect the activity of these starter cultures resulting in starter failure. As there is little information available on the occurrence of bacteriophages in the dairy environment of Kerala, this research communication examines the presence of lytic bacteriophages acting against three potential flavour-producing strains of Lacticaseibacillus paracasei (Lc. paracasei). Dairy effluent samples were screened for the presence of phages against the strains of Lc. paracasei by the multiple host enrichment method. Plates showing clearance zone in spot assay were confirmed for the presence of phages by double-layer agar assay. The plaques obtained in the double-layer agar assay were purified for further identification by next-generation sequencing. A bacteriophage infecting one of the three strains of Lc. paracasei was detected by the plaque assay and the blast annotation of the bacteriophage sequence found 86.05% similarity of the phage to Siphoviridae family. The study endorses the need for monitoring phages in the dairy environment to control phage-related starter failure in the state of Kerala.
Not Available
 
Date 2024-04-29T13:12:00Z
2024-04-29T13:12:00Z
2023-06-21
 
Type Research Paper
 
Identifier Archana, C., Beena, A. K., Murugadas, V., Lijo John, Rajakumar, S. N., Sudheer Babu, Ligimol James and Rahila, M. P. (2023) Detection of lytic phage infecting flavour-producing strain of Lacticaseibacillus paracasei in the dairy effluents of Kerala. Journal of Dairy Research. 90(2): 178-181.
0022-0299
http://krishi.icar.gov.in/jspui/handle/123456789/82432
 
Language English
 
Relation Not Available;
 
Publisher Cambridge University Press