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Comparison of Standard, Fluorogenic and Chromogenic Substrate Based Methods for Enumeration of Escherichia coli in Sasthamkotta Lake of Kerala, India

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Title Comparison of Standard, Fluorogenic and Chromogenic Substrate Based Methods for Enumeration of Escherichia coli in Sasthamkotta Lake of Kerala, India
Not Available
 
Creator Visnuvinayagam, S.
Murugadas, V.
Manasa Das
Radhakrishnan Nair, V.
Sivaraman, G. K.
Toms C. Joseph
 
Subject E. coli
MPN method
Sasthamkotta Lake
EC-MUG Media
chromogenic media
 
Description Not Available
Discovery of chromogenic and fluorogenic substances
has made the conventional method more
hassle-free to detect specific bacteria. A comparative
analysis was conducted to assess the effectiveness
of fluorogenic and chromogenic substrates in aiding
the enumeration of E. coli of Sasthamkotta Lake
located in Kerala, India. The fluorogenic compound,
4-Methyl umbelliferyl-D-glucuronide (MUG) is
employed for the detection of E. coli in food and
water. Similarly, a chromogenic substance, viz., 5-
Bromo-4-chloro-3-indolyl-ß-glucuronic acid (BCIG)
is also employed for E. coli identification in food in
ISO 16649-3. The present study compared the
fluorogenic and chromogenic techniques with the
standard method for enumerating E. coli by the most
probable number (MPN) method. To enumerate the
E. coli, a total of sixteen water samples were
collected from Sasthamkotta Lake of Kerala, India
and analyzed by 5-tube MPN method. Quantitatively,
the results showed that, chromogenic approach
revealed the highest E. coli count, followed
by the fluorogenic and standard methods. Qualitatively,
among the 16 samples, 11 samples were
positive in standard method whereas for other
methods, all 16 samples were positive. Hence it can
be concluded that, in traditional enumeration
technique, there is a substantial chance of under
representation of E. coli by this method. On the other
hand, there is chance of over-representation of E. coli
in the chromogenic and fluorogenic aided methods.
Based on the study, it is suggested that a combination of fluorogenic and chromogenic methods, i.e.,
EC-MUG followed by BCIG plating, would reduce
the false positive or reduce the over representation
of E. coli enumeration.
Not Available
 
Date 2024-04-29T13:49:01Z
2024-04-29T13:49:01Z
2023-07
 
Type Research Paper
 
Identifier Visnuvinayagam, S., Murugadas, V., Manasa Das, Radhakrishnan Nair, V., Sivaraman, G. K. and Toms C. Joseph (2023) Comparison of standard, fluorogenic and chromogenic substrate based methods for enumeration of Escherichia coli in Sasthamkotta lake of Kerala, India. Fish. Techol. 60(3): 206-211.
0015-3001
http://krishi.icar.gov.in/jspui/handle/123456789/82446
 
Language English
 
Relation Not Available;
 
Publisher Society of Fisheries Technologists, India (SOFTI)