Record Details

Fish Scale Derived Hydroxyapatite Incorporated 3D Printed PLA Scaffold for Bone Tissue Engineering

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Fish Scale Derived Hydroxyapatite Incorporated 3D Printed PLA Scaffold for Bone Tissue Engineering
Not Available
 
Creator Nebu George Thomas
Yogesh Bharat Dalvi
Natalia Fijol
Joy Shilpa
Rekha Unni
Binsi, P. K.
Mekha Grace Varghese
Reshmy, R.
Aji Mathew
Anil Sukumaran
 
Subject Not Available
 
Description Not Available
Bone defect repair, particularly in the alveolar region, remains a significant hurdle in periodontics. In recent years, the spotlight in regenerative medicine has fallen on 3D-printed bone scaffolds, especially those constructed of polylactic acid (PLA) infused with hydroxyapatite. This research introduced a novel approach by developing a 3D-printed PLA scaffold enriched with hydroxyapatite derived from fish skin waste (FSHA). Mechanical compression tests revealed that the 3D-printed PLA-FSHA scaffolds had a compressive strength (13.4±5.53 MPa) in the same ballpark as their reference PLA counterparts (20.3±1.08 MPa). Scanning electron micrographs highlighted an average pore size in the scaffold (572 ± 33 µm) conducive to angiogenesis and facilitating cell migration and proliferation. In vitro, cytotoxicity was ascertained using the MTT assay on L929 fibroblast cells. Further in vitro cytocompatibility assessments through actin-DAPI staining and measurements of bone regeneration markers - alkaline phosphatase, osteocalcin and osteopontin-demonstrated that the PLA-FSHA scaffolds not only were biocompatible but also showcased performance on par with the commercial graft, osseograft. This lays the foundation for future in vivo evaluations of bone regenerative capabilities.
Not Available
 
Date 2024-05-13T16:04:39Z
2024-05-13T16:04:39Z
2023-09-08
 
Type Research Paper
 
Identifier Nebu George Thomas, Yogesh Bharat Dalvi, Natalia Fijol, Joy Shilpa, Rekha Unni, Binsi, P. K., Mekha Grace Varghese, Reshmy, R., Aji Mathew and Anil Sukumaran (2023) Fish Scale Derived Hydroxyapatite Incorporated 3D Printed PLA Scaffold for Bone Tissue Engineering. New J. Chem. https://doi.org/10.1039/D3NJ03005A
1144-0546
http://krishi.icar.gov.in/jspui/handle/123456789/82809
 
Language English
 
Relation Not Available;
 
Publisher Royal Society of Chemistry