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Antibacterial efficacy of neem oil fractions on clinical isolates of endometritic cows

Indian Agricultural Research Journals

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Title Antibacterial efficacy of neem oil fractions on clinical isolates of endometritic cows
 
Creator BARMAN, P
YADAV, M C
KUMAR, H
MEUR, S K
RAWAT, M
 
Subject Antibacterial activity
Bacterial isolates
Cows
Endometritis
Neem
 
Description The objective of this study was to assess in vitro antibacterial activity  of neem oil (Azadirachta indica) and its fractions and neem seed aqueous extract on microflora of cervica- vaginal mucus (CVM collected from cows with endometritis.  The CVM samples were obtained from 12 cows during 40-50 days postpartum and were subjected to bacterial isolation and identification. Neem oil was fractionated by 2 approaches, viz. by solvent extraction and by column chromatography using a silica gel column. Neem seed aqueous extract was prepared from dried neem seeds. Isolates (20) were identified, comprising mostly facultative anaerobic bacteria. Non-lactose fermenting  (NLF) bacteria belonging to Enterobacteriaceae family was the most frequently isolated bacteria (25%) followed by Escheriachia coli (20%), Corynebacterium sp. And Streptococcus sp. (15% each), Extraction of neem oil with organic solvents, viz. methanol and hexane yielded 4 fractions which were termed methanol miscrible (F1), methanol-immiscible emulsion (f2), hexane miscrible (F3) and hexane-immiscible emulsion (F4). Column chromatography yielded 3 fractions, viz. F5 (first fraction), F6(Second fraction) and F7 (third fraction). The F1 (methanolic) fraction showed the highest antibacterial activity with a sensitivity pattern of 95% followed by F2 and F3 (85% each), F4 (65%), F6 and F7(40% each). Moreover, the neem seed aqueous extract did not possess any antibacterial activity.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-05-29
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/76386
10.56093/ijans.v79i7.76386
 
Source The Indian Journal of Animal Sciences; Vol. 79 No. 7 (2009)
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/76386/31874
 
Rights Copyright (c) 2017 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0