Record Details

Heat stress amelioration by astaxanthin supplementation and Nrf2 transcribed downregulation of HSP70 and MnSOD in Karan Fries (Holstein Friesian ×Tharparkar) heifers

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Heat stress amelioration by astaxanthin supplementation and Nrf2 transcribed downregulation of HSP70 and MnSOD in Karan Fries (Holstein Friesian ×Tharparkar) heifers
 
Creator KUMAR, SUNIL
SINGH, S V
 
Subject Antioxidant enzymes
Astaxanthin
Gene expression
Karan Fries
Summer stress
 
Description Karan Fries (Holstein Friesian × Tharparkar) heifers are sensitive to heat stress due to exotic inheritance. Therefore their productive performances are affected adversely under tropical climatic conditions. Hence, the present study was conducted on Karan Fries (10-12 months) heifers by dividing into two group i.e. control (fed as per ICAR, 2013) and treatment group (additionally supplemented with astaxanthin @ 0.25 mg/kgBW/day/animal). Environmental parameters (dry and wet bulb temperature, and relative humidity) were recorded throughout the study and temperature humidity index (THI) was calculated. THI was used to assess the levels of summer stress. Blood samples were collected at fortnightly interval from both the groups of heifers for estimation of oxidative stress markers i.e. antioxidants enzyme (SOD, GPx and catalase). RNA was isolated from collected blood samples for Nrf2, HSP70 and MnSOD gene expression at monthly interval. Plasma catalase, SOD and GPx levels were lower in astaxanthin supplemented group of Karan Fries heifers than control group. The expression of HSP70 and MnSOD genes was down-regulated and Nrf2 was up-regulated in treatment compared to control group. The THI had a positive correlation with oxidative stress markers i.e. antioxidants enzyme (SOD, GPx & Catalase) as well as expression pattern of HSP70, and MnSOD genes. Based on the results of the present study, it can be concluded that astaxanthin (potent antioxidant) supplementation was effective in lowering the stress levels in treatment group by down-regulating the genes related to heat stress. Therefore, astaxanthin can be an effective supplement for ameliorating the adverse effect of heat stress, mainly in heat sensitive livestock species.
 
Publisher Indian Council of Agricultural Research
 
Date 2019-12-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/95862
10.56093/ijans.v89i11.95862
 
Source The Indian Journal of Animal Sciences; Vol. 89 No. 11 (2019); 1211–1216
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/95862/38359
 
Rights Copyright (c) 2019 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0