Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa
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Title |
Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa
Not Available |
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Creator |
Roy SC
Atreja SK |
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Subject |
Animal Nutrition
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Description |
Not Available
In the present study attempts were made to detect and quantify the generation of superoxide anion (O(2)( * -)) and hydrogen peroxide (H(2)O(2)) by capacitating buffalo spermatozoa. Ejaculated buffalo spermatozoa were suspended in sp-TALP medium at 50x10(6)mL(-1) and incubated at 38.5 degrees C with 5% CO(2) in air in the absence or presence of heparin (a capacitation inducer) or reduced nicotinamide adenine dinucleotide phosphate (NADPH) or diphenyleneiodonium (DPI, a flavoprotein inhibitor) for 6h. Production rate of O(2)( * -) and H(2)O(2) by spermatozoa at different hours of capacitation were measured by cytochrome c reduction and phenol red oxidation assays, respectively. Spermatozoa generated both O(2)( * -) and H(2)O(2) spontaneously and following stimulation with heparin and a significant increase of O(2)( * -) production was observed in the presence of NADPH. However, DPI inhibited this NADPH-induced O(2)( * -) production and suggested for existence of putative NADPH-oxidase that constitute a specific O(2)( * -) generating systems in buffalo spermatozoa. Results of our study indicated that buffalo spermatozoa generate O(2)( * -) and H(2)O(2) and production of these free radicals is induced during capacitation. Not Available |
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Date |
2024-05-31T11:37:17Z
2024-05-31T11:37:17Z 2008-01-13 |
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Type |
Journal
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Identifier |
Roy SC and Atreja SK (2008) Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa. Animal Reproduction Science. 103:260-270.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/82932 |
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Language |
English
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Relation |
Not Available;
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Publisher |
National center for biotechnology information
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