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Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa

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Title Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa
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Creator Roy SC
Atreja SK
 
Subject Animal Nutrition
 
Description Not Available
In the present study attempts were made to detect and quantify the generation of superoxide anion (O(2)( * -)) and hydrogen peroxide (H(2)O(2)) by capacitating buffalo spermatozoa. Ejaculated buffalo spermatozoa were suspended in sp-TALP medium at 50x10(6)mL(-1) and incubated at 38.5 degrees C with 5% CO(2) in air in the absence or presence of heparin (a capacitation inducer) or reduced nicotinamide adenine dinucleotide phosphate (NADPH) or diphenyleneiodonium (DPI, a flavoprotein inhibitor) for 6h. Production rate of O(2)( * -) and H(2)O(2) by spermatozoa at different hours of capacitation were measured by cytochrome c reduction and phenol red oxidation assays, respectively. Spermatozoa generated both O(2)( * -) and H(2)O(2) spontaneously and following stimulation with heparin and a significant increase of O(2)( * -) production was observed in the presence of NADPH. However, DPI inhibited this NADPH-induced O(2)( * -) production and suggested for existence of putative NADPH-oxidase that constitute a specific O(2)( * -) generating systems in buffalo spermatozoa. Results of our study indicated that buffalo spermatozoa generate O(2)( * -) and H(2)O(2) and production of these free radicals is induced during capacitation.
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Date 2024-05-31T11:37:17Z
2024-05-31T11:37:17Z
2008-01-13
 
Type Journal
 
Identifier Roy SC and Atreja SK (2008) Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa. Animal Reproduction Science. 103:260-270.
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http://krishi.icar.gov.in/jspui/handle/123456789/82932
 
Language English
 
Relation Not Available;
 
Publisher National center for biotechnology information