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Identification of cellulase genes from the metagenomes of Equus burchellifecal samples and functional characterization of a novel bifunctional cellulolytic enzyme.

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Title Identification of cellulase genes from the metagenomes of Equus burchellifecal samples and functional characterization of a novel bifunctional cellulolytic enzyme.
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Creator Chandrasekharaiah M
Thulasi A
Bagath M
Prasanna Kumar D
Singh SS
Sampath KT
 
Subject Exo-1,4-β-glucanase
Avicelase
Metagenome
Enzyme activity
Cloning
 
Description Not Available
The metagenomic approach has been used successfully to isolate novel biocatalyst gene from uncultured microorganisms. The gene encoding exo-1,4-β-glucanase avicelase was amplified from the metagenome of the Equus burchelli fecal sample and cloned. The gene was found to be of 1,007 bp of nucleotide which encodes a protein of 318 amino acids with a calculated MW of 36 kDa. The deduced amino acid sequence was homologous with cellulases belonging to the glycosyl hydrolases 6 superfamily. The expressed protein was active towards the substrates avicel and carboxymethyl cellulose, indicating that it has bifunctional cellulolytic enzyme activity. The recombinant protein showed an activity of 5.23 U with specific activity of 6.8 U mg(-1) protein with the substrate avicel, while when CMC was used, an activity of 3.0 U with a specific activity of 4.2 U mg(-1) protein was achieved. Its optimum pH was determined to be 7.0 and optimum temperature of 35°C.
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Date 2024-05-31T11:56:11Z
2024-05-31T11:56:11Z
2012-01-01
 
Type Journal
 
Identifier Chandrasekharaiah M, Thulasi A, Bagath M, Prasanna Kumar D, Singh SS and Sampath KT. 2012. Identification of cellulase genes from the metagenomes of Equus burchellifecal samples and functional characterization of a novel bifunctional cellulolytic enzyme. Applied Biochemistry and Biotechnology, 167:132-41
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http://krishi.icar.gov.in/jspui/handle/123456789/82962
 
Language English
 
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Publisher Not Available