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Electrophoretic profile of bull sperm membrane proteins as a tool for selection of breeding bull.

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Title Electrophoretic profile of bull sperm membrane proteins as a tool for selection of breeding bull.
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Creator Karunakaran M
Devanathan TG
Jawahar TP
Manimaran K,
Chitra A
Dhali A
Selvaraju S
 
Subject seminal proteins.
in vitro characters
semen
 
Description Not Available
Aim: This study aimed to study the electrophoretic properties of seminal plasma and sperm proteins of Black Bengal buck semen and their correlation with in vitro sperm characters and freezability.

Materials and Methods: Semen ejaculates from nine Black Bengal bucks were collected by artificial vagina (n=20/buck). Ejaculates were evaluated for in vitro sperm characters and electrophoretic profile of seminal protein. In vitro sperm characters were evaluated immediately after collection, after completion of equilibration period, and after freeze-thawing. For seminal protein studies, seminal plasma proteins were precipitated by ice-cold ethanol method, and sperm proteins were extracted by Triton X detergent extraction method. Discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed to assess the molecular weight of seminal proteins. Correlation between in vitro sperm characters and protein bands was determined by Pearson's correlation coefficient, and two-way ANOVA was applied to find the individual buck differences.

Results: Significant difference (p
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Date 2024-05-31T11:59:59Z
2024-05-31T11:59:59Z
2012-01-01
 
Type Journal
 
Identifier Karunakaran M , Devanathan TG , Jawahar TP , Manimaran K, Chitra A, Dhali A and Selvaraju S. 2012. Electrophoretic profile of bull sperm membrane proteins as a tool for selection of breeding bull. Indian Journal of Animal Sciences, 82:1303-1305.
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http://krishi.icar.gov.in/jspui/handle/123456789/82971
 
Language English
 
Relation Not Available;
 
Publisher Not Available