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Effect of vitrification on in vitro matured buffalo oocytes.

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Title Effect of vitrification on in vitro matured buffalo oocytes.
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Creator Mishra A
Chandra V
Sharma GT
 
Subject Goat; In vitro fertilization
In vitro maturation
Oocytes
Vitrification.
 
Description Not Available
Background: Vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. The objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization.

Methods: Oocytes were either cryopreserved as immature cumulus compact (IMCC) (n=98 Exp 1; 102 Exp 2) and immature denuded (IMDN) (n=127 Exp 1; 109 Exp 2) or were first matured in vitro for 28 h and then cryopreserved as mature cumulus compact (MCC) (n=109 Exp 1; 89 Exp 2) or mature denuded (MDN) (n=112 Exp 1; 110 Exp 2) oocytes in four groups. The vitrification solution comprised of Dulbecco's phosphate buffered saline supplemented with 0.5% sucrose, 0.4% bovine serum albumin and 8 M propylene glycol. After 7 days of cryopreservation in liquid nitrogen, oocytes in all groups were evaluated for normal morphologic survival and in vitro maturation (Experiment 1) and fertilization in vitro using epididymal buck spermatozoa (Experiment 2).

Results: The number of oocytes retaining normal morphology was significantly higher (p
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Date 2024-05-31T12:03:29Z
2024-05-31T12:03:29Z
2012-01-01
 
Type Journal
 
Identifier Mishra A, Chandra V and Sharma GT. 2012. Effect of vitrification on in vitro matured buffalo oocytes. Buffalo Bulletin, 31:81-83
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http://krishi.icar.gov.in/jspui/handle/123456789/82978
 
Language English
 
Relation Not Available;
 
Publisher Not Available