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Effect of prostaglandin producing modulators on in vitro growth of buffalo uterine epithelial cells

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Title Effect of prostaglandin producing modulators on in vitro growth of buffalo uterine epithelial cells
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Creator Nandi S
Mondal S
Reddy IJ
 
Subject prostaglandin
in vitro growth of buffalo
uterine epithelial cells
 
Description Not Available
Studies were conducted to examine the effect of seven prostaglandin producing modulators on the in vitro growth of uterine epithelial cells in buffalo. The uterine epithelial cells isolated from slaughtered buffaloes were cultured in media containing (a) Lipopolysaccaride (LPS): 0, 0.01, 0.1, 1, 10 and 100 μg/ml, (b) linoleic acid: 0, 0.01, 0.1, 1, 10 and 100 μg/ml, (c) linolenic acid: 0, 0.01, 0.1, 1, 10 and 100 μg/ml, (d) oxytocin: 0, 10, 100, 1,000, 10,000 and 100,000 nm, (e) tumor necrosis factor-α (TNF-α): 0, 0.05, 0.5, 1, 2.5 and 5 nm, (f) progesterone: 0.1, 10, 25, 50, 75 and 100 nM, and (g) estradiol: 0, 2.5, 5, 10, 20 and 50 nM. The control medium consisted of RPMI-1640 plus 10% bovine fetal serum. The growth of uterine epithelial were measured in terms of viability, cell number increment and monolayer formation. Results suggested that the growth of uterine epithelial cells were significantly (P < 0.05) higher in media containing 10 μg/ml, 10 μg/ml, 1 nm and 10 μg/ml linoleic acid, linolenic acid, TNF-α and LPS, respectively compared to control and lower doses used. Progesterone, estradiol and oxytocin did not significantly (P > 0.05) increase the growth of uterine epithelial cells. In conclusion, the growth of uterine epithelial cells increased when exposed to modulators in the order of linoleic acid ≥ linolenic acid ≥ LPS ≥ TNF-α > progesterone > estrogen > oxytocin.
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Date 2024-05-31T12:04:17Z
2024-05-31T12:04:17Z
2012-01-01
 
Type Journal
 
Identifier Nandi S, Mondal S and Reddy IJ. 2012. Effect of prostaglandin producing modulators on in vitro growth of buffalo uterine epithelial cells . Theriogenology, 77:1014-1020.
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http://krishi.icar.gov.in/jspui/handle/123456789/82980
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Ltd