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Evaluation of sperm functional attributes in relation to in vitro sperm zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality

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Title Evaluation of sperm functional attributes in relation to in vitro sperm zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality
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Creator Selvaraju S
Ravindra JP
Ghosh J
Gupta PSP
Suresh KP
 
Subject Animal Nutrition
 
Description Not Available
The objective of this study was to evaluate sperm functional attributes in relation to in vitro sperm-zona binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality. Frozen-thawed forty-eight ejaculates from eight Surti buffalo bulls (six ejaculates/bull) obtained by artificial vagina were used. Frozen semen from each bull was thawed, pooled, and subjected for sperm functional (six replicates) and in vitro fertilization (four replicates) tests. The progressive forward motility, plasmalemma functional integrity assessed by fluorogenic [6-carboxyfluorescein diacetate (CFDA), and propidium iodide (PI)], hypoosmotic swelling (HOS), and hypoosmotic swelling-Giemsa (HOS-G) test, mitochondrial membrane potential, sperm nuclear morphology, the number of sperm bound to zona and cleavage rate differed significantly (P40% cleavage rate, n=5, and group II,
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Date 2024-05-31T12:26:03Z
2024-05-31T12:26:03Z
2008-07
 
Type Journal
 
Identifier Selvaraju S, Ravindra JP, Ghosh J, Gupta PSP, Suresh KP (2008). Evaluation of sperm functional attributes in relation to in vitro sperm zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality, Animal Reproduction Science, 106: 311-321
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http://krishi.icar.gov.in/jspui/handle/123456789/83000
 
Language English
 
Relation Not Available;
 
Publisher National center for biotechnology information