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Superoxide dismutase and oxygen transport mechanism in endemic fish Delminichthys ghetaldii (Steindachner, 1882) under mild hypoxia

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Title Superoxide dismutase and oxygen transport mechanism in endemic fish Delminichthys ghetaldii (Steindachner, 1882) under mild hypoxia
 
Creator Friščić, Jasna
Dekić, Radoslav
Ivanc, Aleksandar
Kukavica, Biljana
 
Subject Antioxidant enzymes
Karst
Popovo minnow
Reactive oxygen species
Water pollution
 
Description 410-415
Hypoxia, a condition when dissolved oxygen (DO) drops down below 2 mg/L1, is one of the most common stressors in
aquatic environment. Aquatic animals often overcome this condition with a combination of physiological, behavioural,
biochemical and molecular responses. In the present study, we examined the effect of moderate hypoxia on superoxide
dismutase (SOD) activity and oxygen transport mechanism in Delminichthys ghetaldii erythrocytes. Popovo minnow
(Delminichthys ghetaldii) is an endemic cyprinid fish which inhabits springs and streams in fields Ljubomir, Dabar and
Fatnica in B&H, and Bacina lakes in Croatia. During autumn and spring floods, Popovo minnows emerge from the
underground waters to surface estavelle. Withdrawal of the water to the underground basins brings them back to their
underground habitat. Fishes were collected in the flood plain of Fatnica field (430053″N; 181924″E). Following
erythrocyte lineage parameters were measured: number of red blood cells (RBC), hemoglobin concentration, Packed cell
volume (PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin Concentration (MCHC) and MCH
(Mean Corpuscular Hemoglobin). Also, the characterization of the SOD from RBC is presented for the first time. Native
PAGE resolved the presence of three CuZn SOD isoforms, while isoelectrofocusing revealed the presence of two anionic
SOD isoforms with pI 3.4 and 3.5. Exposure to conditions of mild hypoxia induced changes in fish SOD activity,
hemoglobin concentration and MCH. Results indicate Delminichthys ghetaldii responds to hypoxia by enhancement of the
oxygen carrying capacity and triggering SOD activity.
 
Date 2024-05-31T09:31:42Z
2024-05-31T09:31:42Z
2024-06
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64014
https://doi.org/10.56042/ijeb.v62i06.2605
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(06) [June 2024]