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A high-grain protein content locus on barley (Hordeum vulgare) chromosome 6 is associated with increased flag leaf proteolysis and nitrogen remobilization

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Title A high-grain protein content locus on barley (Hordeum vulgare) chromosome 6 is associated with increased flag leaf proteolysis and nitrogen remobilization
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Creator Aravind K. Jukanti† and Andreas M. Fischer
 
Subject Barley, proteolysis, grain protein content
 
Description Not Available
Leaf senescence and nitrogen remobilization from senescing tissues are two
important factors determining grain protein content (GPC) in cereals. We
compared near - isogenic barley (Hordeum vulgare L.) germplasm varying in the
allelic state of a major GPC quantitative trait locus on chromosome 6,
delineated by molecular markers HVM74 and ABG458 and explaining
approximately 46% of the variability in this trait. High GPC was consistently
associated with earlier whole - plant senescence. SDS–PAGE and immunoblot
analysis of flag leaf proteins indicated earlier leaf protein [including ribulose -
1,5 - bisphosphate carboxylase/oxygenase (Rubisco)] degradation in high - GPC
germplasm. This was accompanied by enhanced availability of ammonium
and glutamine in developing kernels, suggesting increased phloem retranslocation
of nitrogen. Based on previous microarray analysis, we performed
a detailed expression study of six leaf genes, tentatively involved in plastidial
proteolysis, vacuolar proteolysis, intermediary N metabolism and N transport.
All of these were upregulated in high - GPC barley, mostly around 21 to 28 days
past anthesis, prior to or around the time demonstrating maximal differences in
leaf protein (including Rubisco) levels. Therefore, these genes represent
potential targets to manipulate grain protein accumulation. It appears likely
that their functional analysis will enhance our understanding of whole - plant N
recycling. Additionally, earlier leaf (photosynthetic) protein degradation may
lead to reduced N carbon assimilation in high - GPC germplasm, explaining
past studies demonstrating a negative correlation between GPC and yield.
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Date 2024-06-11T12:27:33Z
2024-06-11T12:27:33Z
2007-11-20
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/83504
 
Language English
 
Relation Not Available;
 
Publisher Not Available