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Rapid Detection of Rice Tungro Spherical Virus by RT-PCR and Dot Blot Hybridization

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Title Rapid Detection of Rice Tungro Spherical Virus by RT-PCR and Dot Blot Hybridization
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Creator S. K. Mangrauthia, P. Malathi, D. Krishnaveni, C. S. Reddy, B. C. Viraktamath, S. M. Balachandran, C. N. Neeraja and A. K. Biswal
 
Subject RTSV, diagnosis, disease, nucleic acid
 
Description Not Available
Tungro disease of rice is very difficult to identify in the field, as characteristic symptoms are not expressed in all
varieties. Moreover, physiological disorders mimic the disease symptoms quite often and make it more complex for
diagnosis. In the present work, Rice tungro spherical virus (RTSV) was detected in diseased samples using sequence
specific primers and probes. The disease symptoms observed were stunting, yellow to orange discolouration and
twisting of leaf tips. A two step reverse transcription polymerase chain reaction (RT-PCR) and RNA dot blot
hybridization methods were used to detect RTSV in rice plants. Total RNA was extracted from symptomatic and
non-symptomatic rice leaf samples and was used to amplify the specific sequences of RTSV. Primers were designed
based on the flanking regions of the coat protein gene sequences available in NCBI database. The same amplicon
was cloned and further used as a probe to detect the virus through RNA-dot blot hybridization. Both the methods of
diagnosis were demonstrated to be very specific in identifying the virus in diseased samples. As eight of the 26
tungro suspected but non-symptomatic leaf samples were diagnosed positive for RTSV, it confirmed further that
viral symptoms do not correspond to the viral titer always.
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Date 2024-06-11T15:51:58Z
2024-06-11T15:51:58Z
2010-09-27
 
Type Research Paper
 
Identifier Mangrauthia SK, P Malathi, D Krishnaveni, CS Reddy, BC Viraktamath, SM Balachandran, CN Neeraja and AK Biswal. 2010. Rapid detection of Rice Tungro Spherical Virus by RT-PCR and Dot Blot Hybridization. Journal of Mycology and Plant Pathology. 40:445-449
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http://krishi.icar.gov.in/jspui/handle/123456789/83615
 
Language English
 
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Publisher Not Available