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Production, Optimization, Partial Purification, and Characterization of a Novel Cellulase from Bacillus subtilis 171ES and Its Potential for Use in Textiles

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Title Production, Optimization, Partial Purification, and Characterization of a Novel Cellulase from Bacillus subtilis 171ES and Its Potential for Use in Textiles
 
Creator Msakni, Sarra
Demirkan, Elif
Tanik, Nilay Aladag
 
Subject Biostoning
Enzyme
Optimization
Thin layer chromatography
Vegetative impurities
 
Description 677-687
One hundred Bacillus sp. strains isolated from different soils were screened for cellulase production. A strain that
produced maximum cellulase amounts was selected and identified by 16S rRNA analysis and was named Bacillus subtilis
171ES. In order to quantitatively determine the enzyme production capacity of the new isolate, it was grown in 3 different
media. Optimization studies were carried out in the best medium. The results of nutritional optimization in the best liquid
media showed that the best nitrogen and carbon sources, and metal ions were found to be milk powder, maltose and LiSO4,
respectively. In the study, enzyme production increased 6.6 times in the newly created modified medium. The enzyme was
partially purified and characterized. The Optimum pH and temperature were 7 and 50°C, respectively. The BaCl2 and
MnSO4 and ions were effective on activity. The kinetic values of the enzyme were Vmax 63.7 U/mL and Km 0.0064 mM. The
molecular weight was estimated as 63 kDa. The potential of the enzyme for biostoning on denim fabric and removal of the
cellulosic or vegetative impurities on wool fabric was evaluated. It was also found to be effective in fabrics. The cellulase
enzyme of the new 171ES strain has the potential to be used in various textile processes.
 
Date 2024-06-07T09:24:20Z
2024-06-07T09:24:20Z
2024-06
 
Type Article
 
Identifier 0022-4456 (Print); 0975-1084 (Online)
http://nopr.niscpr.res.in/handle/123456789/64040
https://doi.org/10.56042/jsir.v83i6.4165
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source JSIR Vol.83(6) [June 2024]