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Carrier frequency of autosomal recessive disorders CVM and DUMPS in Sahiwal and Holstein Friesian cattle in Chhattisgarh

Indian Agricultural Research Journals

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Title Carrier frequency of autosomal recessive disorders CVM and DUMPS in Sahiwal and Holstein Friesian cattle in Chhattisgarh
 
Creator KUMAR, VINEET
JAIN, ASIT
JAIN, TRIPTI
MUKHERJEE, KISHORE
RATHORE, AAKANKSHA
PARVEEN, KAISER
ANSARI, FARHEEN
 
Subject CVM, DUMPS, Genetic disorders
 
Description In the present study, genetic screening of Sahiwal and Holstein Friesian (HF) cattle and its crosses was conducted using the PCR-RFLP technique to detect the presence of CVM and DUMPS disorders. Genomic DNA was extracted from 50 Sahiwal and 100 HF animals and its crosses using commercially available kits. Gene-specific primers were utilized for in vitro amplification of SLC35A3 and UMPS genes by PCR, followed by RFLP analysis using PstI and AvaI restriction endonucleases for CVM and DUMPS, respectively. Subsequently, the PCR products were purified and sequenced to confirm any mutations in the DNA target sequences. Notably, no affected or carrier animals for CVM and DUMPS disorders were identified among the screened animals. Thus, the gene and genotype frequency of CVM and DUMPS carriers was zero, indicating a 100% frequency of normal animals. The study underscores the importance of continued screening for these genetic disorders, involving a larger sample size across the state, and advocates for mandatory screening before utilizing animals for breeding purposes, in adherence to minimum standard protocols.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-06-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/132934
10.56093/ijans.v94i6.132934
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 6 (2024); 564–567
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/132934/54804
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0