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Genetic and antigenic characterization of bluetongue virus serotype-1 isolated from goat in India

Indian Agricultural Research Journals

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Title Genetic and antigenic characterization of bluetongue virus serotype-1 isolated from goat in India
 
Creator CHAND, KARAM
SUBRAMANIAM, SARAVANAN
BISWAS, SANCHAY KUMAR
GAUTAM, SIDDHARTH
KHARAYAT, NITISH SINGH
SHARMA, GAURAV KUMAR
 
Subject Antigenic relationship, Bluetongue virus, BTV-1 serotype, Goat, Next generation sequencing, Phylogeny
 
Description Bluetongue virus (BTV) affects domestic and wild ruminants and is transmitted by Culicoides spp. In the present study, genetic and antigenic characterization of the BTV-1 serotype (MKD20/08/Ind isolate) isolated from goat was carried out to understand the phylogenetic relationship and molecular epidemiology of bluetongue. Full genome sequencing of BTV-1 isolate was carried out using next-generation sequencing (NGS). The sequence data was analyzed and the phylogenetic relationship of the goat isolate with other BTV-1 was established. Phylogenetic analysis grouped the MKD-20/08/Ind isolate within BTV serotype 1, with a high level of nucleotide sequence identity (>99%) to the previous BTV-1, confirming its serotype. The phylogenetic tree constructed from the outer capsid protein genes, seg-2 and seg-6, segregated MKD-20/08/Ind with other Indian BTV-1 isolates reported earlier. All BTV genome segments were found to be evolving under strong purifying (negative) selection. The mean substitution rate of different segments of BTV-1 isolates varied from 1.663×10-2 (seg-8) to 3.949×10-3 (seg-1) substitutions per site per year. The time to the most recent common ancestor (tMRCA) indicates all the genome segments of BTV-1 might have an older ancestor. The phenotypic antigenic relationship (r) of the MKD20/08/Ind isolate with other BTV-1 isolate-specific hyperimmune serum (HIS) determined by serum neutralization test (SNT) was 0.44 to 0.80. The neutralization behaviours of the MKD20/08/Ind with other BTV-1 isolate-specific HIS suggest probable minor subtype antigenic variation. Full-genome sequencing and phylogenetic analysis of BTV-1/MKD-20/08/Ind isolate indicate ancestral relationships amongst the Indian and Mediterranean BTV-1 isolates.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-06-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/144563
10.56093/ijans.v94i6.144563
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 6 (2024); 487–491
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/144563/54781
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0