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Expression of recombinant classical swine fever virus E2 glycoprotein instable High Five cells

Indian Agricultural Research Journals

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Title Expression of recombinant classical swine fever virus E2 glycoprotein instable High Five cells
 
Creator BHARDWAJ, EKTA
DEKA, DIPAK
VERMA, RAMNEEK
 
Subject Classical swine fever virus (CSFV), Dot blot, Insect cell expression, Western blot
 
Description Classical swine fever is one of the most important viral diseases of domestic pigs and wild boar. It is a notifiable disease to the World Organization for Animal Health. In the present study, codon optimized CSFV E2 gene was cloned into a laboratory-modified insect cell expression vector for producing the recombinant protein in secretory form. Endotoxin-free recombinant plasmid prepared from the positive clone was transfected into the High Five insect cell line and then selected against zeocin antibiotic for the development of a stable cell line. The stable High Five cell line successfully secreted E2 recombinant protein with a molecular weight of ~65 kDa as revealed by SDS-PAGE and Western blot. The recombinant E2 protein showed efficient reactivity with known CSFV anti-serum and monoclonal antibodies (MAbs against E2) in dot blot assay indicating its potential as a diagnostic antigen.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-06-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/145934
10.56093/ijans.v94i6.145934
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 6 (2024); 522–526
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/145934/54794
https://epubs.icar.org.in/index.php/IJAnS/article/view/145934/54795
 
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https://creativecommons.org/licenses/by-nc-sa/4.0