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IN VITRO VALIDATION OF SEX SORTED BULL SEMEN PRODUCED USING FLOW CYTOMETRIC METHOD

Indian Agricultural Research Journals

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Title IN VITRO VALIDATION OF SEX SORTED BULL SEMEN PRODUCED USING FLOW CYTOMETRIC METHOD
 
Creator S. Rangasamy
S. Balasubramanian
G. Dhinakar Raj
A. Palanisammi
D. Reena
R. Chitra
 
Subject Bull
Sex sorted semen and flow cytometry
 
Description A commercially procured flow cytometrically sorted semen was reanalyzed to validate the effect of flow cytometry on structural and functional viability of spermatozoa. Flow cytometric analysis of commercially procured sex sorted frozen thawed bull semen showed the percentage of X sperms to be 95.31± 0.21 and Y sperms to be 4.69 ± 0.27, respectively. As expected sexed sperm had lower post thaw motility than the non-sexed sperm. However, no significant differences in the percentage of sperms with intact acrosome and expression of ROS were observed. Interestingly, DNA fragmentation index was statistically lower for the commercially procured sorted than for unsorted frozen thawed bull semen. 
 
Publisher Tamil Nadu Veterinary and Animal Sciences University
 
Date 2024-06-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJVASR/article/view/152726
 
Source Indian Journal of Veterinary and Animal Sciences Research; Vol. 53 No. 2 (2024): IJVASR March - April 2024; 84-89
2347-2774
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJVASR/article/view/152726/54786
 
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