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Studies on a new antimicrobial peptide from Vibrio proteolyticus MT110

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Studies on a new antimicrobial peptide from Vibrio proteolyticus MT110
 
Creator Verma, Himanshu
Mihooliya, Kanti N.
Nandal, Jitender
Sahoo, Debendra K.
 
Subject QR Microbiology
 
Description The marine environment is known for its vast diversity of the microbial population; however, less explored for bioactive compounds. In this study, an AMP produced by a new marine isolate, Vibrio proteolyticus MT110, showed broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria. The AMP was purified to homogeneity using ethyl acetate extraction followed by RP-HPLC, and LC-MS analysis showed its molecular weight as 980 Da. The MIC of AMP (peptide-MT110) was obtained in the 7.81-31.25 µg/mL range against different indicator strains. Peptide-MT110 showed stability of its antimicrobial activity at 15-121 °C and pH 4-10 and in the presence of various hydrolytic enzymes. The peaks at 1536 cm-1 and 1712 cm-1 wavenumbers in FTIR spectra confirmed the peptidic nature of AMP, and its amino acid analysis confirmed the presence of tyrosine and isoleucine. The antibacterial activity of peptide-MT110 is confirmed by PI assay and TEM. The optimization of peptide-MT110 production using statistical methods resulted in a 2.64-fold higher production. The physicochemical properties and stability in wide pH and temperature ranges showed the potential of peptide-MT110 for its development as a drug candidate. This is believed to be the first report on an AMP from Vibrio proteolyticus.
 
Publisher TAYLOR AND FRANCIS
 
Date 2024-02
 
Type Article
PeerReviewed
 
Relation https://www.tandfonline.com/doi/full/10.1080/10826068.2023.2209892
http://crdd.osdd.net/open/3107/
 
Identifier Verma, Himanshu and Mihooliya, Kanti N. and Nandal, Jitender and Sahoo, Debendra K. (2024) Studies on a new antimicrobial peptide from Vibrio proteolyticus MT110. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 54 (2). pp. 193-206.