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Analysis of Culturable Bacterial Diversity of Pangong Tso Lake via a 16S rRNA Tag Sequencing Approach

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Analysis of Culturable Bacterial Diversity of Pangong Tso Lake via a 16S rRNA Tag Sequencing Approach
 
Creator Yadav, Pooja
Das, Joyasree
Sundharam, Shiva S.
Krishnamurthi, Srinivasan
 
Subject QR Microbiology
 
Description The Pangong Tso lake is a high-altitude freshwater habitat wherein the resident microbes experience unique selective pressures, i.e., high radiation, low nutrient content, desiccation, and temperature extremes. Our study attempts to analyze the diversity of culturable bacteria by applying a high-throughput amplicon sequencing approach based on long read technology to determine the spectrum of bacterial diversity supported by axenic media. The phyla Pseudomonadota, Bacteriodetes, and Actinomycetota were retrieved as the predominant taxa in both water and sediment samples. The genera Hydrogenophaga and Rheinheimera, Pseudomonas, Loktanella, Marinomonas, and Flavobacterium were abundantly present in the sediment and water samples, respectively. Low nutrient conditions supported the growth of taxa within the phyla Bacteriodetes, Actinomycetota, and Cyanobacteria and were biased towards the selection of Pseudomonas, Hydrogenophaga, Bacillus, and Enterococcus spp. Our study recommends that media formulations can be finalized after analyzing culturable diversity through a high-throughput sequencing effort to retrieve maximum species diversity targeting novel/relevant taxa.
 
Publisher MDPI
 
Date 2024-02
 
Type Article
PeerReviewed
 
Relation https://www.mdpi.com/2076-2607/12/2/397
http://crdd.osdd.net/open/3108/
 
Identifier Yadav, Pooja and Das, Joyasree and Sundharam, Shiva S. and Krishnamurthi, Srinivasan (2024) Analysis of Culturable Bacterial Diversity of Pangong Tso Lake via a 16S rRNA Tag Sequencing Approach. MICROORGANISMS, 12 (2).