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Production of polyclonal antibodies for detection of Nucleopolyhedrovirus infecting Helicoverpa armigera

OAR@ICRISAT

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Relation http://oar.icrisat.org/882/
 
Title Production of polyclonal antibodies for detection of Nucleopolyhedrovirus infecting Helicoverpa armigera
 
Creator Kumar, C S
Sireesha, K
Ranga Rao, G V
Reddy, A S
Waliyar, F
Rambabu, C
Kumar, P L
 
Subject Agriculture-Farming, Production, Technology, Economics
 
Description Polyclonal antibodies were raised against purified polyhedrin [polyocclusion body (POB)] protein preparations of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) and were used to develop a direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) to detect HaNPV. The sensitivity of the DAC-ELISA was 15 ng/ml of partially purified viral protein or 30 ng/ml POBs from the HaNPV infected larval extracts. The antibodies were highly specific to polyhedrin protein of HaNPV and were useful to diagnose NPV at early stages of larval infection and also for the quantification of the NPVs during production of viral insecticides.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/882/1/Indian_Journal_of_Plant_Protection_35_2_210-212_2007.pdf
Kumar, C S and Sireesha, K and Ranga Rao, G V and Reddy, A S and Waliyar, F and Rambabu, C and Kumar, P L (2007) Production of polyclonal antibodies for detection of Nucleopolyhedrovirus infecting Helicoverpa armigera. Indian Journal of Plant Protection, 35 (2). pp. 210-212.