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Bioproduction of yeast single cell oil with acute oral toxicity study intended for edible oil application

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Bioproduction of yeast single cell oil with acute oral toxicity study intended for edible oil application
 
Creator Nautiyal, Abhilek K.
Priyanka, .
Paul, Pranoy
Raut, Sachin
Bhaskar, Thallada
Chowdhury, Nilotpal
Khatri, Neeraj
Ghosh, Debashish
 
Subject QR Microbiology
 
Description Human nutrition and health rely on edible oils. Global demand for edible oils is expanding, necessitating the discovery of new natural oil sources subjected to adequate quality and safety evaluation. However, in contrast to other agricultural products, India's edible oil supply is surprisingly dependent on imports. The microbial oil is generated by fermentation of oleaginous yeast Rhodotorula mucilaginosa IIPL32 MTCC 25056 using biodiesel plant byproduct crude glycerol as a fermentable carbon source. Enriched with monounsaturated fatty acid, nutritional indices mapping based on the fatty acid composition of the yeast SCO, suggested its plausible use as an edible oil blend. In the present study, acute toxicity evaluation of the yeast SCO in C57BL/6 mice has been performed by randomly dividing the animals into 5 groups with 50, 300, 2000, and 5000 mg/Kg yeast SCO dosage, respectively, and predicted the median lethal dose (LD50). Detailed blood biochemistry and kidney and liver histopathology analyses were also reported. The functions of the liver enzymes were also evaluated to check and confirm the anticipated toxicity. To determine cell viability and in vitro biocompatibility, the 3T3-L1 cell line and haemolysis tests were performed. The results suggested the plausible use of yeast SCO as an edible oil blend due to its non-toxic nature in mice models.
 
Publisher SPRINGER
 
Date 2024-07
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s11274-024-03976-2
http://crdd.osdd.net/open/3130/
 
Identifier Nautiyal, Abhilek K. and Priyanka, . and Paul, Pranoy and Raut, Sachin and Bhaskar, Thallada and Chowdhury, Nilotpal and Khatri, Neeraj and Ghosh, Debashish (2024) Bioproduction of yeast single cell oil with acute oral toxicity study intended for edible oil application. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 40 (7).