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High-density bin-based genetic map reveals a 530-kb chromosome segment derived from wild peanut contributing to late leaf spot resistance

OAR@ICRISAT

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Relation http://oar.icrisat.org/12726/
https://link.springer.com/article/10.1007/s00122-024-04580-6
https://doi.org/10.1007/s00122-024-04580-6
 
Title High-density bin-based genetic map reveals a 530-kb chromosome segment derived from wild peanut contributing to late leaf spot resistance
 
Creator Pan, J
Li, X
Fu, C
Bian, J
Wang, Z
Yu, C
Liu, X
Wang, G
Tian, R
Song, X
Li, C
Xia, H
Zhao, S
Hou, L
Gao, M
Zi, H
Bertioli, D
Leal-Bertioli, S
Pandey, M K
Wang, X
Zhao, C
 
Subject Groundnut
Genetics and Genomics
 
Description Late leaf spot (LLS) is one of the major foliar diseases of peanut, causing serious yield loss and affecting the quality of kernel and forage. Some wild Arachis species possess higher resistance to LLS as compared with cultivated peanut; however, ploidy level differences restrict utilization of wild species. In this study, a synthetic amphidiploid (Ipadur) of wild peanuts with high LLS resistance was used to cross with Tifrunner to construct TI population. In total, 200 recombinant inbred lines were collected for whole-genome resequencing. A high-density bin-based genetic linkage map was constructed, which includes 4,809 bin markers with an average inter-bin distance of 0.43 cM. The recombination across cultivated and wild species was unevenly distributed, providing a novel recombination landscape for cultivated-wild Arachis species. Using phenotyping data collected across three environments, 28 QTLs for LLS disease resistance were identified, explaining 4.35–20.42% of phenotypic variation. The major QTL located on chromosome 14, qLLS14.1, could be consistently detected in 2021 Jiyang and 2022 Henan with 20.42% and 12.12% PVE, respectively. A favorable 530-kb chromosome segment derived from Ipadur was identified in the region of qLLS14.1, in which 23 disease resistance proteins were located and six of them showed significant sequence variations between Tifrunner and Ipadur. Allelic variation analysis indicating the 530-kb segment of wild species might contribute to the disease resistance of LLS. These associate genomic regions and candidate resistance genes are of great significance for peanut breeding programs for bringing durable resistance through pyramiding such multiple LLS resistance loci into peanut cultivars.
 
Publisher Springer
 
Date 2024-03-05
 
Type Article
PeerReviewed
 
Identifier Pan, J and Li, X and Fu, C and Bian, J and Wang, Z and Yu, C and Liu, X and Wang, G and Tian, R and Song, X and Li, C and Xia, H and Zhao, S and Hou, L and Gao, M and Zi, H and Bertioli, D and Leal-Bertioli, S and Pandey, M K and Wang, X and Zhao, C (2024) High-density bin-based genetic map reveals a 530-kb chromosome segment derived from wild peanut contributing to late leaf spot resistance. Theoretical and Applied Genetics, 137. ISSN 0040-5752