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Characterization of DhHal3p: A moderately thermostable FMN-Binding Flavoprotein with biomedical potential from halotolerant yeast, Debaryomyces hansenii using partial structure prediction

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Title Characterization of DhHal3p: A moderately thermostable FMN-Binding Flavoprotein with biomedical potential from halotolerant yeast, Debaryomyces hansenii using partial structure prediction
 
Creator Sharma, Aditi
Singh, Rashmi
Kumari, Arzoo
Puri, Sanjeev
Arya, Shailendra Kumar
Sachdeva, Ruchi
Minhas, Anu Priya
 
Subject Debaryomyces hansenii
Decarboxylase
Docking
Flavoprotein
PPCDC
DhHal3p
 
Description 430-442
Enzymes within the CoA biosynthetic pathway are highlighted in the literature as promising targets for antimicrobial drugs, beyond their role in metabolism. In Saccharomyces cerevisiae, the model yeast, the Hal3 protein is a pivotal component of the PPCDC complex, a critical enzyme involved in the fourth step of Coenzyme A (CoA) biosynthetic pathway. Characterizing such proteins from extremophilic strains might present their substantial therapeutic potential. Therefore, the focus of the present study was to identify and characterize the putative DhHal3 gene and encoded protein from Debaryomyces hansenii, a halotolerant and teleomorph of commensal yeast, Candida famata. DhHal3 encoded a 559 amino acids peptide with a unique 48 amino acids aspartic acid-rich C-terminal domain. DhHal3p was identified as a flavoprotein (PFAM ID "PF02441") with conserved PLXANTand PXMNXXMW motifs, and H344 residue involved in FMN binding. Heterogeneously expressed 6xHistidine- tagged DhHal3p appeared as ~73.37kDa protein on SDS-PAGE, exhibiting pyruvate decarboxylation activity(V0 = 0.57 units/mL) in vitro. Thermo-profiling and circular dichroism (CD) analysis suggested DhHal3p is amoderately thermostable FMN-binding flavoprotein from D. hansenii. Docking simulations supported stronginteractions between DhHal3p structure and FMN (binding energy = -4.04 kcal/mol). Further investigation into thefunctional characteristics of DhHal3p could yield pivotal insights into a variety of cellular processes, paving the wayfor therapeutic interventions.
 
Date 2024-06-26T10:41:30Z
2024-06-26T10:41:30Z
2024-07
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/64094
https://doi.org/10.56042/ijbb.v61i7.4367
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.61(07) [July 2024]