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Development, characterization and utilization of microsatellite markers in pigeonpea

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Relation http://oar.icrisat.org/912/
http://dx.doi.org/10.1111/j.1439-0523.2007.01324.x
 
Title Development, characterization and utilization of microsatellite markers in pigeonpea
 
Creator Odeny, D A
Jayashree, B
Ferguson, M
Hoisington, D A
Crouch, J H
Gebhard, C
 
Subject Pigeonpea
 
Description Pigeonpea is a major legume of the semi-arid tropics that has been neglected in terms of molecular breeding. The objectives of this study were to develop microsatellite markers and evaluate their potential for use in pigeonpea genetics and breeding. Two hundred and eight microsatellite loci were isolated by screening a non-enriched partial genomic library. Primers were designed for 39 microsatellite loci, 20 of which amplified polymerase chain reaction products of the expected size. Nineteen of the primer pairs were polymorphic amongst 15 cultivated and nine wild pigeonpea accessions providing evidence for cross-species transferability within the genus Cajanus. A total of 98 alleles were detected at the 19 polymorphic loci with an average of 4.9 alleles per locus. The observed heterozygosity ranged from 0.17 to 0.80 with a mean of 0.42 per locus. Less allelic variation (31 alleles) was observed within the cultivated species than across the wild species (92 alleles). The diversity analysis readily distinguished all wild relatives from each other and from the cultivated germplasm. Development of more microsatellites is recommended for future genomic studies in pigeonpea.
 
Publisher Blackwell Publishing
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/912/1/Plant_Breeding_126_130-136_2007.pdf
Odeny, D A and Jayashree, B and Ferguson, M and Hoisington, D A and Crouch, J H and Gebhard, C (2007) Development, characterization and utilization of microsatellite markers in pigeonpea. Plant Breeding, 126 (2). pp. 130-136.