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<p class="MDPI13authornames">Dynamic monitoring of the cytotoxic effects of <em>Cousinia iconica</em> extracts on A549 and RL95-2 cells using the xCELLigence RTCA system</p>

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Title Statement <p class="MDPI13authornames">Dynamic monitoring of the cytotoxic effects of <em>Cousinia iconica</em> extracts on A549 and RL95-2 cells using the xCELLigence RTCA system</p>
 
Added Entry - Uncontrolled Name Paşayeva, Leyla
Erciyes University Scientific Research Projects Coordinating Unit (project number THD-2018-7921)
 
Uncontrolled Index Term Asteraceae; Cytotoxicity; LC-MS/MS; RL95-2; xCELLigence.
 
Summary, etc. <p>Asteraceae family plants have potential anticancer activity. The plants have various pharmacological effects and the effects of <em>Cousinia iconica</em> Hub. - Mor. (Asteraceae) depend on many factors, mainly on the type of cells and dose.  In this study, the cytotoxic effects of the <em>C. iconica</em> were investigated using different tumor cell lines: Non-small lung cancer (A549) and human endometrium carcinoma cell line (RL95-2). For the study, cell viability was determined with MTT assay and the xCELLigence Real‑Time Cell Analyser (RTCA) SP instrument was used. Different concentrations of <em>C. iconica</em> extract and sub-extracts were applied on A549 and RL95-2 cell lines.  The ethyl acetate sub-extract and methanol extract of <em>C. iconica</em> were cytotoxic to RL95-2 cells. According to RTCA results, the IC<sub>50</sub> values of ethyl acetate sub-extract 44.82, 60.89, and 55.22 µg/mL and methanol extract 265.52, 250.48, and 246.65 µg/mL, for 24, 48, and 72-h exposure, respectively. Real-time xCELLigence measurements show that RL95-2 cells are more sensitive to ethyl acetate sub-extract than methanol extract. The results show that ethyl acetate sub-extract has a cytotoxic effect on RL95-2 endometrium cancer cells, with low IC<sub>50</sub> values close to cisplatin. This study is the known first study to show the cytotoxic effects of <em>C. iconica</em> extracts on both A549 and RL95-2 cell lines.</p>
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2024-06-28 11:25:10
 
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http://op.niscair.res.in/index.php/IJTK/article/view/48665
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 23, ##issue.no## 6 (2024): Indian Journal of Traditional Knowledge
 
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