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Enhancing the plant growth promoting activity of Trichoderma viride biopesticide using substrate supplementation and co-cultivation

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Title Enhancing the plant growth promoting activity of Trichoderma viride biopesticide using substrate supplementation and co-cultivation
 
Creator Narwade, JD
Odaneth, AA
Lele, SS
 
Subject Biocontrol
Biofungicide
Biopesticides
Capsicum frutescens
Corn cobs
Plant-growth promotion
Solid-state fermentation
 
Description 593-603
Substrate supplementation and co-cultivation approaches were used to enhance activity of biofungicide Trichoderma viride.
Biopesticides were produced using corn cobs (T), corn cobs + 1% keratin (K), corn cobs + 5% lignin (L), and corn cobs + 1%
keratin + 5% lignin (K+L). Also, biopesticides were produced through co-culturing with Bacillus subtilis MTCC8142, by
sequential inoculation (B+T) and simultaneous inoculation (T3+B2) (B2+T3). The highest spore count was produced by
(T3+B2) of 6.25×108 spores/g of substrate, followed by (T) of 6.20×108 spores/g of substrate. GC-MS characterization of ethyl
acetate extract of biopesticides revealed different metabolites produced under different conditions. The zone of inhibition of
extract was greatest for (T) of 21 mm, followed by (K) of 20.7 mm and for (B2+T3) of 18 mm at 10 mg/mL concentration. (K)
and (B2+T3) did not affect antifungal properties; however, other substrate-supplemented and co-cultivated biopesticides
decreased them. Competition between two microbes prevented (T3+B2) from producing antimicrobial metabolites; hence, no
zone of inhibition was observed. The biopesticides (K), (L) and (B2+T3) significantly enhanced growth of chili plants
(Capsicum frutescens L.), outperforming other biopesticides. The production of phthalates, also known as phytotoxins, by B+T
resulted in a decrease in plant growth. Adding 1% keratin to corn cobs (K) and (B2+T3) sequential inoculation are best ways to
boost plant growth promoting activity of T. viride without affecting its biocontrol activity.
 
Date 2024-07-09T10:22:18Z
2024-07-09T10:22:18Z
2024-07
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64221
https://doi.org/10.56042/ijeb.v62i07.12087
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(07) [July 2024]