Record Details

Pro-angiogenic and wound healing potential of baicalein: In vitro, in ovo and in silico evaluation

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Pro-angiogenic and wound healing potential of baicalein: In vitro, in ovo and in silico evaluation
 
Creator Mathew, Krupa Ann
Bernadette, KM
John, Annie
Helen, A
 
Subject Angiogenesis
Chinese skullcap
Docking
Huangqin
Hypoxia
Scutellaria baicalensis
 
Description 540-552
Angiogenesis is critical for the repair of damaged or diseased tissues. Hypoxia is a major factor promoting angiogenesis
in physiological conditions. Baicalein, a flavone derived from the Chinese skullcap or Huangqin, Scutellaria baicalensis
Georgi which is known to exhibit hypoxia mimetic activity. Here, we investigated the pro-angiogenic and wound healing
potential of baicalein in vitro, in ovo, and in silico with a view to develop green wound dressings. The human keratinocyte
cell line, HaCaT, was subjected to baicalein at varied concentrations in vitro and assessed for cell viability, cell cycle, and
apoptosis induction. The study showed that lower concentrations of baicalein (below 10 μM) promoted HaCaT proliferation,
while higher concentrations induced apoptosis. Baicalein at low concentrations also promoted angiogenesis, as evidenced by
the CAM (Chorioallantoic membrane) assay performed on chicken eggs. Gene expression analysis of Keratin 10 also
suggested the activation of keratinocytes, leading to augmented wound healing after baicalein treatment. The in silico
docking experiments reflect the potential of baicalein to promote angiogenesis via a HIF-independent pathway involving the
agonistic transcriptional activation of the ERRα-PGC1α complex. The results highlight the capability of baicalein to
promote angiogenesis and wound healing and thus hold potential for the development of angiogenesis-promoting scaffolds
and wound dressings.
 
Date 2024-07-09T10:33:30Z
2024-07-09T10:33:30Z
2024-07
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64227
https://doi.org/10.56042/ijeb.v62i07.12083
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(07) [July 2024]