Record Details

Invitro inhibition of Daboia russelii (Shaw & Nodder) venom with alginic acid-based silver nanoparticles

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Invitro inhibition of Daboia russelii (Shaw & Nodder) venom with alginic acid-based silver nanoparticles
 
Creator Saha, Kanchan
Parua, Poulami
Sarkhel, Sumana
Jamal, Nuzhat Ara
 
Subject Alginic acid
Direct hemolytic assay
Proteolytic activity
Russell’s viper
Silver nanoparticle
nakebite envenoming
 
Description 504-514
Envenomation by Russell’s viper [Daboia russelii (Shaw & Nodder, 1797)] is a major medical emergency in tropical
countries. The antivenom therapy is a conventional remedy for such medical emergency, but it has limitations and side
effects. Nanomedicine and nanotechnology are the most prospective areas of research in the current scenario. In the present
study, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of crudeDaboia russelli venom
(VRV) was performed. Alginic acid-based silver nanoparticles (AgNP-ALG) were synthesized and characterized using
UV-Visible spectroscopy, Dynamic Light Scattering (DLS), Scanning electron microscope (SEM), and X-ray diffraction
analysis (XRD). SNPs have average hydrodynamic size of 80.30 nm with 0.271 PDI. X-ray diffraction analysis of
AgNP-ALG, which confirmed the cubic crystal shape of silver. SEM studies of AgNP-ALG showed particle sizes ranging
from 10 to 50 nm. Spectroscopic analysis showed a decrease in the absorbance intensity of venom upon interaction with
AgNP-ALG, indicating interaction with venom proteins. From the data available from fluorescence spectroscopy,
it is evident that viper venom preincubated with AgNP-ALG causes quenching of fluorescence intensity. The results
obtained by direct hemolytic assay,proteolytic activity and blood clotting test revealed venom action inhibition due to silver
nanoparticles. Thus, in the present study we have emphasized that silver nanoparticles inhibit the action of Daboia russelli
venom in vitro.
 
Date 2024-07-09T10:40:11Z
2024-07-09T10:40:11Z
2024-07
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64231
https://doi.org/10.56042/ijeb.v62i07.12069
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(07) [July 2024]