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Differential expression and genetic polymorphism of DMRT1 and FOXL2 genes in the gonads of Kadaknath chicken

Indian Agricultural Research Journals

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Title Differential expression and genetic polymorphism of DMRT1 and FOXL2 genes in the gonads of Kadaknath chicken
 
Creator BACHAMOLLA, SHIVANI
SIVALINGAM, JAYAKUMAR
CHINNIPREETAM, VELPULA
KRISHNA, DAIDA
MURUGESAN, SHANMUGAM
REDDY, B RAJITH
BHATTACHARYA, TARUN KUMAR
ULLENGALA, RAJKUMAR
 
Subject Chicken, DMRT1, FOXL2, Genetic polymorphism, Ovary, Relative gene expression
 
Description In chicken, the left ovary is functional and the right ovary becomes rudimentary in the later stage of the embryonic development. The expression and genetic polymorphism of the DMRT1 and FOXL2 genes are assessed in order to understand the distinct developing process of gonads in male and female embryonic gonads. In the current study, 19th day Kadaknath chicken gonadal tissue samples were collected from the embryos and RT-PCR analysis and DNA sequencing was done. According to the expression studies, the left ovaries relative expression of FOXL2 was 2.14 and 12.65 factor greater than that of the right ovaries and testes, while the right testis relative expression of DMRT1 was 3.78 and 3.83 fold higher than that of the left and right ovaries. DNA sequence analysis in the left ovary identified a nonsynonymous alteration (c.289C>G) involving histidine to aspartic acid at the 289th position in the FOXL2 coding region. The 51st position of exon 6 of DMRT1 has a G→A transition SNP that is not part of the coding sequence (CDS). Males showed higher levels of the DMRT1 gene than females, while females expressed higher levels of FOXL2. It was found that the DMRT1 and FOXL2 genes are polymorphic. The genetic variability in the gonads and the differences in DMRT1 and FOXL2 gene expression allow learning more about the critical mechanisms underlying chicken gonadal development.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-07-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/149461
10.56093/ijans.v94i7.149461
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 7 (2024); 620–625
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/149461/55108
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0