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Techniques for diagnosis of pseudomonas solanacearum, and for resistance screening against groundnut bacterial wilt

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Title Techniques for diagnosis of pseudomonas solanacearum, and for resistance screening against groundnut bacterial wilt
 
Creator Mehan, V K
McDonald, D
 
Subject Groundnut
 
Description Bacterial wilt , caused by pseudomonas solanacearum, is a widespread and destructive disease of such economically important crops as banana, eggplant, groundnut, pepper, potato, tobacco, and tomato , in the tropics, subtropics, and warm temperate regions. The pathogen has a wide host range and an exceptional ability to survive in the roots of nonhost plants and in the soil. This manual presents techniques for detection, isolation, and identification of all variants of the wilt pathogen. The use of enzyme-l inked immunosor bent assay (ELISA) to detect the bacterium in plant tissues, seed, and soil is emphasized. DNA-based diagnostics for P. solanacearum are described, as are an infectivity titration technique to determine the virulence of the wilt pathogen, and several inoculation techniques used in evaluat ing host-plant resistance to groundnut bacterial wilt.
 
Publisher International Crops Research Institute for the Semi-Arid Tropics
 
Date 1995
 
Type Monograph
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1025/1/RA_000280.pdf
Mehan, V K and McDonald, D (1995) Techniques for diagnosis of pseudomonas solanacearum, and for resistance screening against groundnut bacterial wilt. Technical Report. International Crops Research Institute for the Semi-Arid Tropics.