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Substitution of carbonate by non-physiological synergistic anion modulates the stability and iron release kinetics of serum transferrin.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Substitution of carbonate by non-physiological synergistic anion modulates the stability and iron release kinetics of serum transferrin.
 
Creator Kumar, Rajesh
Sharma, Deepak
Kumar, Navinder
Kumari, Beeta
Shabnam, .
Kumar, Sanjeev
Kumar, Rajesh
 
Subject QR Microbiology
 
Description Serum transferrin (sTf) is a bi-lobal protein. Each lobe of sTf binds one Fe3+ ion in the presence of a synergistic anion. Physiologically, carbonate is the main synergistic anion but other anions such as oxalate, malonate, glycolate, maleate, glycine, etc. can substitute for carbonate in vitro. The present work provides the possible pathways by which the substitution of carbonate with oxalate affects the structural, kinetic, thermodynamic, and functional properties of blood plasma sTf. Analysis of equilibrium experiments measuring iron release and structural unfolding of carbonate and oxalate bound diferric-sTf (Fe2sTf) as a function of pH, urea concentration, and temperature reveal that the structural and iron-centers stability of Fe2sTf increase by substitution of carbonate with oxalate. Analysis of isothermal titration calorimetry (ITC) scans showed that the affinity of Fe3+ with apo-sTf is enhanced by substituting carbonate with oxalate. Analysis of kinetic and thermodynamic parameters measured for the iron release from the carbonate and oxalate bound monoferric-N-lobe of sTf (FeNsTf) and Fe2sTf at pH 7.4 and pH 5.6 reveals that the substitution of carbonate with oxalate inhibits/retards the iron release via increasing the enthalpic barriers.
 
Publisher ELSEVIER
 
Date 2022-10
 
Type Article
PeerReviewed
 
Relation https://www.sciencedirect.com/science/article/pii/S1570963922001030?via%3Dihub
http://crdd.osdd.net/open/3151/
 
Identifier Kumar, Rajesh and Sharma, Deepak and Kumar, Navinder and Kumari, Beeta and Shabnam, . and Kumar, Sanjeev and Kumar, Rajesh (2022) Substitution of carbonate by non-physiological synergistic anion modulates the stability and iron release kinetics of serum transferrin. Biochimica et biophysica acta, 1871 (1). ISSN 0006-3002