Deciphering the Role of S-adenosyl Homocysteine Nucleosidase in Quorum Sensing Mediated Biofilm Formation
DIR@IMTECH: CSIR-Institute of Microbial Technology
View Archive InfoField | Value | |
Title |
Deciphering the Role of S-adenosyl Homocysteine Nucleosidase in Quorum Sensing Mediated Biofilm Formation
|
|
Creator |
Yadav, Jyoti
Das, Satyajeet Singh, Suraj Jyoti, Anupam Srivastava , Vijay Kumar Sharma, Vinay Kumar, Sanjit Kumar, Sujeet Kaushik , Sanket |
|
Subject |
QR Microbiology
|
|
Description |
S-adenosylhomocysteine nucleosidase (MTAN) is a protein that plays a crucial role in several pathways of bacteria that are essential for its survival and pathogenesis. In addition to the role of MTAN in methyl-transfer reactions, methionine biosynthesis, and polyamine synthesis, MTAN is also involved in bacterial quorum sensing (QS). In QS, chemical signaling autoinducer (AI) secreted by bacteria assists cell to cell communication and is regulated in a cell density-dependent manner. They play a significant role in the formation of bacterial biofilm. MTAN plays a major role in the synthesis of these autoinducers. Signaling molecules secreted by bacteria, i.e., AI-1 are recognized as acylated homoserine lactones (AHL) that function as signaling molecules within bacteria. QS enables bacteria to establish physical interactions leading to biofilm formation. The formation of biofilm is a primary reason for the development of multidrug-resistant properties in pathogenic bacteria like Enterococcus faecalis (E. faecalis). In this regard, inhibition of E. faecalis MTAN (EfMTAN) will block the QS and alter the bacterial biofilm formation. In addition to this, it will also block methionine biosynthesis and many other critical metabolic processes. It should also be noted that inhibition of EfMTAN will not have any effect on human beings as this enzyme is not present in humans. This review provides a comprehensive overview of the structural-functional relationship of MTAN. We have also highlighted the current status, enigmas that warrant further studies, and the prospects for identifying potential inhibitors of EfMTAN for the treatment of E. faecalis infections. In addition to this, we have also reported structural studies of EfMTAN using homology modeling and highlighted the putative binding sites of the protein.
|
|
Publisher |
BENTHAM SCIENCE
|
|
Date |
2022-06-15
|
|
Type |
Article
PeerReviewed |
|
Relation |
https://www.eurekaselect.com/article/123780
http://crdd.osdd.net/open/3157/ |
|
Identifier |
Yadav, Jyoti and Das, Satyajeet and Singh, Suraj and Jyoti, Anupam and Srivastava , Vijay Kumar and Sharma, Vinay and Kumar, Sanjit and Kumar, Sujeet and Kaushik , Sanket (2022) Deciphering the Role of S-adenosyl Homocysteine Nucleosidase in Quorum Sensing Mediated Biofilm Formation. Current protein & peptide science, 23 (4). pp. 211-225. ISSN 1389-2037
|
|