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Multifaceted personality and roles of heme enzymes in industrial biotechnology

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Multifaceted personality and roles of heme enzymes in industrial biotechnology
 
Creator Bhardwaj , Mahipal
Kamble , Pranay
Mundhe , Priyanka
Jindal, Monika
Thakur, Payal
Bajaj, Priyanka
 
Subject QR Microbiology
 
Description Heme enzymes are the most prominent category of iron-containing metalloenzymes with the capability of catalyzing an astonishingly wide range of reactions like epoxidation, hydroxylation, demethylation, desaturation, reduction, sulfoxidation, and decarboxylation. Various enzymes in this category are P450s, heme peroxidases, catalases, myoglobin, cytochrome C, and others. Besides this, the natural promiscuity and amenability of these enzymes to protein engineering and evolution have also added several non-native reactions such as C-H, N-H, S-H insertions, cyclopropanation, and other industrially important reactions to their capabilities. Surprisingly, all of these reactions and their wide substrate scopes are attributed to changes in the active site scaffold of different heme enzymes as the center of all enzymes is constituted by a porphyrin ring containing iron. Multiple prominent research groups across the world, including 2018, Nobel Laureate Frances Arnold's group, have shown keen interest in engineering and evolving these enzymes for utilizing their industrial potential. Besides engineering the active site, researchers have also explored the possibility of these enzymes catalyzing non-native reactions by replacing the center porphyrin ring with other cofactors or by changing the iron in the porphyrin ring with other metal ions along with engineering the active site and thereby creating novel artificial metalloenzymes. Thus, in this mini-review from our group, for the first time, we are trying to catalog various activities catalyzed by heme enzymes and their engineered variants and their active usage in various industries along with shedding light on their potential for use in various applications in the future.
 
Publisher SPRINGER LINK
 
Date 2023-12
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s13205-023-03804-8
http://crdd.osdd.net/open/3165/
 
Identifier Bhardwaj , Mahipal and Kamble , Pranay and Mundhe , Priyanka and Jindal, Monika and Thakur, Payal and Bajaj, Priyanka (2023) Multifaceted personality and roles of heme enzymes in industrial biotechnology. 3 Biotech, 13 (389). ISSN 2190-572X